Raybould J N, Barro T, Sawadogo R, Mordzifa F
Tropenmed Parasitol. 1982 Jun;33(2):87-93.
Laboratory rearing of Simulium damnosum complex species enables female adults to be indirectly identified from their larval progeny. A simple, readily transportable rearing apparatus was developed for investigations by the Onchocerciasis Control programme by using unbreakable, easily packed and reassembled parts and by feeding the larvae at least partly with living green algae (Chlorophyceae) cultured in the laboratory. Although the larvae are sensitive to excess nitrogen compounds, the use of algae and a large volume of water obviated the need for a separate purification system in which water was circulated by an A/C electrical centrifugal pump between a container and an inclined trough in which most of the larvae developed. The rate of development was rapid giving rise to full size larvae (suitable for cytotaxonomic determination), pupae and adults. The adults were attracted into a collecting device by light. Developments to protect the larvae during electrical power failures included a slow-drying wooden rearing trough and an automatic switch to an alternative D/C power supply and pump. This technique has been in general use in O.C.P. laboratories in Benin, Ghana, Ivory Coast and Upper Volta and many thousands of larvae have been reared.
对恶蚋复合种进行实验室饲养，能够从其幼虫后代中间接鉴定出成年雌虫。盘尾丝虫病防治计划开发了一种简单、便于运输的饲养装置用于研究，该装置采用不易破碎、易于打包和重新组装的部件，并至少部分用实验室培养的活绿藻（绿藻纲）喂养幼虫。虽然幼虫对过量的含氮化合物敏感，但使用藻类和大量的水使得无需单独的净化系统，在该净化系统中，水由空调电动离心泵在一个容器和一个倾斜水槽之间循环，大部分幼虫在该倾斜水槽中发育。发育速度很快，能培育出全尺寸的幼虫（适合进行细胞分类学鉴定）、蛹和成虫。成虫会被光吸引到收集装置中。在停电期间保护幼虫的改进措施包括一个缓慢干燥的木制饲养槽以及自动切换到备用直流电源和泵。这项技术已在贝宁、加纳、象牙海岸和上沃尔特的盘尾丝虫病防治计划实验室中普遍使用，并且已经饲养了数千只幼虫。
