Chojkier M, Groszmann R J
Am J Physiol. 1981 May;240(5):G371-5. doi: 10.1152/ajpgi.1981.240.5.G371.
A method for determining the extent of portal-systemic shunting (PSS) is described and evaluated in lightly anesthetized (ketamine) rats. This method relies on the intrasplenic injection of 15-micrometers gamma-labeled spheres and the subsequent measurement of liver and lung radioactivities. After the splenic injection of the microspheres, a fraction of microspheres shunting the hepatic sinusoidal bed will be entrapped in the lungs. Thus, the ratio, lung/(lung + liver) radioactivities, will indicate the degree of PSS. The percent of PSS was determined in normal (n = 13; 1.2 +/- 0.3%), in cirrhotic (n = 9; 15.0 +/- 4.5%; P less than 0.01), and in portal vein-ligated animals (n = 14; 95.0 +/- 0.9%; P less than 0.001 for normal and cirrhotic). In addition, PSS was sequentially quantitated (n = 3) before (3.0%) and after (89%) partial portal vein ligation. The variability of PSS found in cirrhotic rats (0.7-41.0%) is in agreement with the reported data for PSS in patients. This technique, which is simple, rapid, and reproducible, allows the quantitation of PSS in small laboratory animals.
本文描述并评估了一种在轻度麻醉（氯胺酮）大鼠中测定门体分流（PSS）程度的方法。该方法依赖于经脾内注射15微米的γ标记微球，随后测量肝脏和肺部的放射性。在脾内注射微球后，一部分分流肝血窦床的微球会滞留在肺部。因此，肺/（肺 + 肝）放射性的比值将指示PSS的程度。测定了正常大鼠（n = 13；1.2 +/- 0.3%）、肝硬化大鼠（n = 9；15.0 +/- 4.5%；P < 0.01）和门静脉结扎动物（n = 14；95.0 +/- 0.9%；与正常和肝硬化大鼠相比，P < 0.001）的PSS百分比。此外，对部分门静脉结扎前（3.0%）和结扎后（89%）的PSS进行了连续定量（n = 3）。在肝硬化大鼠中发现的PSS变异性（0.7 - 41.0%）与报道的患者PSS数据一致。这种技术简单、快速且可重复，能够对小型实验动物的PSS进行定量。
