登革病毒在鼠类细胞培养物中的增殖

Propagation of dengue viruses in murine cell cultures.

作者信息

Koff W, Elm J L, Halstead S B

出版信息

Am J Trop Med Hyg. 1981 Mar;30(2):461-4. doi: 10.4269/ajtmh.1981.30.461.

DOI:10.4269/ajtmh.1981.30.461

Abstract

Dengue viruses were cultivated in Balb 3T12-3 cells, a continuous line of cells derived from mouse embryo cell cultures. Optimal serum concentrations were determined for maximal kinetics of virus replication. All four types of dengue viruses replicate in these cells, and peak virus titers were obtained by 72 hours post-infection. The successful growth of dengue viruses in a continuous line of mouse cells should allow for more extensive studies examining the cellular immune response to dengue virus infection in murine models.

摘要

登革病毒在Balb 3T12 - 3细胞中培养，该细胞系源自小鼠胚胎细胞培养物。确定了病毒复制最大动力学的最佳血清浓度。所有四种类型的登革病毒都在这些细胞中复制，感染后72小时获得病毒滴度峰值。登革病毒在小鼠连续细胞系中的成功生长应有助于在小鼠模型中更广泛地研究对登革病毒感染的细胞免疫反应。