登革2型病毒在无血清培养基的幼仓鼠肾细胞中的培养。
Cultivation of dengue virus type 2 in baby hamster kidney cells in serum-free medium.
作者信息
Malewicz B, Jenkin H M
出版信息
Am J Trop Med Hyg. 1979 Sep;28(5):918-20.
PMID:484777
Abstract
Propagation of dengue virus type 2 (New Guinea C strain) was performed using a shaker culture of baby hamster kidney cells (BHK-21) cultivated in serum-free modified Waymouth medium. Maximum virus titer varied from 10(8.3) to 10(8.8) plaque forming units/ml after incubation of BHK-21 cells in suspension culture at 37 dgrees C for 40-48 hours post-infection.
摘要
登革2型病毒（新几内亚C株）的增殖是通过在无血清改良韦茅斯培养基中培养的幼仓鼠肾细胞（BHK-21）摇瓶培养来进行的。在感染后于37℃将BHK-21细胞进行悬浮培养40 - 48小时后，最大病毒滴度在10(8.3)至10(8.8)蚀斑形成单位/毫升之间变化。
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