用氚水通过同位素交换法制备氚标记的洋红霉素和柔红霉素及其在水溶液中标记稳定性的测定
[Preparation of tritium-labelled carminomycin and rubomycin by the isotope-exchange method with tritiated water and the determination of the label stability in aqueous solutions].
作者信息
Karpov V L, Dedenkov A N
出版信息
Antibiotiki. 1981 Apr;26(4):257-8.
A simple procedure for preparation of tritiated anthracycline antibiotics, such as rubomycin and carminomycin is described. The method is based on isotope exchange with tritiated water at 100 degrees C. The specific activity of the preparations is several hundreds decays a minute per 1 microgram. The stability of the antibiotic label in aqueous solutions at various pH values was determined. At pH 7.2 and a temperature of 37 degrees C the loss of the tritium by the end of the 1st, 2nd, 3rd, 4th and 7th day amounted to 7.1, 11, 14, 19, and 24 per cent, respectively. It is concluded tht tritiated antibiotics prepared with the method of tritiated water exchange may be used in biomedical investigations.
本文描述了一种制备氚标记蒽环类抗生素（如柔红霉素和洋红霉素）的简单方法。该方法基于在100℃下与氚水进行同位素交换。制剂的比活度为每1微克每分钟数百次衰变。测定了抗生素标记物在不同pH值水溶液中的稳定性。在pH 7.2和37℃温度下，第1、2、3、4和7天结束时氚的损失分别为7.1%、11%、14%、19%和24%。得出结论，用氚水交换法制备的氚标记抗生素可用于生物医学研究。
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