Sone S, Fidler I J
Cancer Res. 1981 Jun;41(6):2401-6.
The purpose of this study was to determine whether the presence of progressively growing pulmonary metastases influences the number and function of alveolar macrophages (AM). Female F344 rats were given i.v. injections of cells from a metastatic variant line of the syngeneic adenocarcinoma MADB-105. At Days 7, 14, 21, and 28 after injection, normal and tumor-bearing animals (3/group) were killed, and their AM were harvested by lavage. The functional integrity of AM was determined by their capacity to phagocytose opsonized erythrocytes and by their ability to respond to a variety of activating agents in vitro. Normal and metastasis-bearing rats were given i.v. injections of Nocardia rubra cell wall skeleton to determine whether the presence of large pulmonary metastases would interfere with AM activation in situ. The data demonstrated that the presence of progressively growing lung metastases led to a slight increase in the number of harvested AM and that these cells from tumor-bearing rats were as phagocytic as AM obtained from normal rats. Also, AM harvested from rats with metastases became tumoricidal against syngeneic tumor target cells in response to activation stimuli in vitro and in vivo. We conclude that, in this tumor system, the presence of progressively growing lung metastases doses not suppress the number or function of AM. The use of agents known to activate AM could, therefore, be of benefit in the therapy of pulmonary metastases.
本研究的目的是确定逐渐生长的肺转移瘤的存在是否会影响肺泡巨噬细胞（AM）的数量和功能。给雌性F344大鼠静脉注射同基因腺癌MADB - 105转移变异株的细胞。在注射后的第7、14、21和28天，处死正常和荷瘤动物（每组3只），通过灌洗收集它们的AM。通过AM吞噬调理红细胞的能力以及它们在体外对多种激活剂的反应能力来确定AM的功能完整性。给正常和荷转移瘤的大鼠静脉注射红色诺卡氏菌细胞壁骨架，以确定大量肺转移瘤的存在是否会干扰AM的原位激活。数据表明，逐渐生长的肺转移瘤的存在导致收获的AM数量略有增加，并且这些来自荷瘤大鼠的细胞与从正常大鼠获得的AM一样具有吞噬作用。此外，从有转移瘤的大鼠中收获的AM在体外和体内对激活刺激有反应时，对同基因肿瘤靶细胞具有杀瘤作用。我们得出结论，在这个肿瘤系统中，逐渐生长的肺转移瘤的存在不会抑制AM的数量或功能。因此，使用已知能激活AM的药物可能对肺转移瘤的治疗有益。
