Tachibana K, Sone S, Tsubura E, Kishino Y
Br J Cancer. 1984 Mar;49(3):343-8. doi: 10.1038/bjc.1984.54.
F344 rats were given saline, vitamin A placebo or vitamin A analogues orally for 4 consecutive days. The following day they were killed and their alveolar macrophages (AM phi) were harvested by lavage. The functional integrity of the AM phi was determined by their capacity to phagocytize opsonized SRBC and to kill syngeneic adenocarcinoma cell lines nonspecifically. Results showed that 4 days treatment with greater than 100 IU of vitamin A as retinyl palmitate per gram body weight rendered the AM phi tumoricidal against syngeneic mammary adenocarcinoma cell lines (MADB-100 and MADB-200) and that AM phi activated with retinyl palmitate showed increased ability to phagocytize opsonized SRBC. Other retinoids, such as retinoic acid and retinol, had the same effect of inducing tumoricidal activity in rat AM phi. AM phi harvested from normal rats were also rendered tumoricidal by direct interaction with greater than 10(3) IU ml-1 of retinyl palmitate for 24 h in vitro. Thus, vitamin A at high doses can increase the phagocytic and tumoricidal activities of rat AM phi.
给F344大鼠连续4天口服生理盐水、维生素A安慰剂或维生素A类似物。次日将它们处死,通过灌洗收集其肺泡巨噬细胞(AM phi)。通过AM phi吞噬调理的SRBC以及非特异性杀伤同基因腺癌细胞系的能力来确定AM phi的功能完整性。结果显示,以每克体重大于100 IU的棕榈酸视黄酯形式的维生素A进行4天治疗,可使AM phi对同基因乳腺腺癌细胞系(MADB - 100和MADB - 200)具有杀肿瘤活性,并且用棕榈酸视黄酯激活的AM phi吞噬调理的SRBC的能力增强。其他类维生素A,如视黄酸和视黄醇,在大鼠AM phi中具有相同的诱导杀肿瘤活性的作用。从正常大鼠收集的AM phi在体外与大于10(3) IU/ml的棕榈酸视黄酯直接相互作用24小时后也具有杀肿瘤活性。因此,高剂量的维生素A可增强大鼠AM phi的吞噬和杀肿瘤活性。