Minami S A, Collis P S, Young E E, Weeks D P
Cell. 1981 Apr;24(1):89-95. doi: 10.1016/0092-8674(81)90504-3.
Flagellar excision in Chlamydomonas reinhardii triggers a rapid and extensive induction of tubulin synthesis. Cloned plasmids, pFT beta 1 and pFT beta 2, carrying cDNA inserts complementary to beta-tubulin mRNA, have been prepared and used to demonstrate a direct requirement for tubulin mRNA synthesis during tubulin induction. Increased tubulin mRNA synthesis is detected within 5 min after deflagellation. During the 45 min peak period of tubulin synthesis, tubulin mRNA accumulates to levels 15- to 35-fold higher than those found in control (non-deflagellated) cells. In addition, there appears to be a direct correlation between tubulin mRNA concentrations and the levels of tubulin production during the induction and deinduction cycle that accompanies flagellar regeneration. Amiprophosmethyl (APM), a compound we reported earlier as a selective inhibitor of tubulin synthesis in deflagellated cells, is shown to block the accumulation of tubulin mRNA following flagellar excision and to cause the rapid loss of tubulin mRNA from cells treated at the peak of induction.
莱茵衣藻鞭毛切除引发微管蛋白合成的快速且广泛诱导。已制备携带与β-微管蛋白mRNA互补的cDNA插入片段的克隆质粒pFTβ1和pFTβ2，并用于证明在微管蛋白诱导过程中对微管蛋白mRNA合成的直接需求。去鞭毛后5分钟内即可检测到微管蛋白mRNA合成增加。在微管蛋白合成的45分钟高峰期，微管蛋白mRNA积累至比对照（未去鞭毛）细胞中高15至35倍的水平。此外，在鞭毛再生伴随的诱导和去诱导循环过程中，微管蛋白mRNA浓度与微管蛋白产生水平之间似乎存在直接相关性。我们之前报道的作为去鞭毛细胞中微管蛋白合成选择性抑制剂的胺丙畏（APM），被证明可阻断鞭毛切除后微管蛋白mRNA的积累，并导致在诱导高峰期处理的细胞中微管蛋白mRNA迅速丢失。
