Collis P S, Weeks D P
Science. 1978 Oct 27;202(4366):440-2. doi: 10.1126/science.568309.
Amiprophos methyl (APM) is a strong, readily reversible and highly selective inhibitor of tubulin synthesis in Chlamydomonas reinhardi. The extensive induction of tubulin synthesis that accompanies flagellar regeneration in this organism is prevented by 3 to 10 micrometerAPM. When applied after induction has begun, APM causes a rapid cessation of tubulin synthesis. Translation studies in vitro indicate that the lack of tubulin production in APM-treated cells is not due to a direct inhibition of tubulin messenger RNA translation but rather to a selective depletion of tubulin messenger RNA.
甲基胺草磷（APM）是莱茵衣藻微管蛋白合成的一种强效、易于逆转且高度选择性的抑制剂。在这种生物体中，鞭毛再生时伴随的微管蛋白合成的广泛诱导会被3至10微米的APM阻止。当在诱导开始后应用时，APM会导致微管蛋白合成迅速停止。体外翻译研究表明，经APM处理的细胞中微管蛋白产量的缺乏并非由于微管蛋白信使核糖核酸翻译的直接抑制，而是由于微管蛋白信使核糖核酸的选择性耗竭。
