Vatassery G T, Sheridan M A, Krezowski A M, Divine A S, Bach H L
Clin Biochem. 1981 Feb;14(1):21-4. doi: 10.1016/0009-9120(81)90120-x.
Adult human cerebrospinal fluid (CSF) was assayed for total lipid levels by the sulfo-phospho-vanillin color reaction. Lipids in a 1-2 ml sample of CSF are extracted with a 2:1 chlofoform-methanol mixture and the solvent extract, after equilibration with 0.74 percent potassium chloride, is evaporated to dryness. The lipid in the residue is estimated by the sulfo-phospho-vanillin reaction. The extraction process is essentially quantitative for lipid mixtures normally found in CF. The technique has an average coefficient of variation of 1.5 percent and the recovery of added lipids is nearly quantitative. It can be used as a rapid routine method for estimating CSF total lipids and several samples can be processed simultaneously. Estimations of total lipid levels using the sulfo-phospho-vanillin reaction agree quite well with the results obtained by densitometric quantitation of charred lipids. A preliminary analysis of 22 samples of adult human CSF containing normal levels of total protein gave an average total lipid level of 0.78 (+/- 0.07 SEM) mg/dl. Total lipid levels in CSF did not significantly correlate with age. However lipid levels in CSF show a significant positive correlation with CSF total protein levels.
采用磺基磷香草醛显色反应测定成人脑脊液（CSF）中的总脂质水平。取1 - 2毫升脑脊液样本，用氯仿 - 甲醇混合液（2:1）萃取其中的脂质，萃取后的溶剂提取物与0.74%的氯化钾平衡后，蒸发至干。用磺基磷香草醛反应测定残渣中的脂质。对于脑脊液中通常存在的脂质混合物，该提取过程基本是定量的。该技术的平均变异系数为1.5%，添加脂质的回收率几乎是定量的。它可作为一种快速常规方法用于估计脑脊液总脂质，并且可以同时处理多个样本。使用磺基磷香草醛反应测定的总脂质水平与通过烧焦脂质的光密度定量获得的结果相当吻合。对22份总蛋白水平正常的成人脑脊液样本进行初步分析，得出平均总脂质水平为0.78（±0.07 SEM）毫克/分升。脑脊液中的总脂质水平与年龄无显著相关性。然而，脑脊液中的脂质水平与脑脊液总蛋白水平呈显著正相关。
