Cheng Yu-Shen, Zheng Yi, VanderGheynst Jean S
Department of Biological and Agricultural Engineering, University of California, Davis, CA 95616, USA.
Lipids. 2011 Jan;46(1):95-103. doi: 10.1007/s11745-010-3494-0. Epub 2010 Nov 11.
A colorimetric sulfo-phospho-vanillin (SPV) method was developed for high throughput analysis of total lipids. The developed method uses a reaction mixture that is maintained in a 96-well microplate throughout the entire assay. The new assay provides the following advantages over other methods of lipid measurement: (1) background absorbance can be easily corrected for each well, (2) there is less risk of handling and transferring sulfuric acid contained in reaction mixtures, (3) color develops more consistently providing more accurate measurement of absorbance, and (4) the assay can be used for quantitative measurement of lipids extracted from a wide variety of sources. Unlike other spectrophotometric approaches that use fluorescent dyes, the optimal spectra and reaction conditions for the developed assay do not vary with the sample source. The developed method was used to measure lipids in extracts from four strains of microalgae. No significant difference was found in lipid determination when lipid content was measured using the new method and compared to results obtained using a macro-gravimetric method.
开发了一种用于总脂质高通量分析的比色磺基磷香草醛（SPV）方法。所开发的方法使用一种反应混合物，在整个测定过程中保持在96孔微孔板中。与其他脂质测量方法相比，新方法具有以下优点：（1）可以轻松校正每个孔的背景吸光度；（2）处理和转移反应混合物中所含硫酸的风险较小；（3）显色更一致，吸光度测量更准确；（4）该测定法可用于定量测量从多种来源提取的脂质。与其他使用荧光染料的分光光度法不同，所开发测定法的最佳光谱和反应条件不会因样品来源而异。所开发的方法用于测量四种微藻菌株提取物中的脂质。使用新方法测量脂质含量并与使用宏观重量法获得的结果进行比较时，在脂质测定中未发现显著差异。
