Baine R M, Brown H G
Clin Chem. 1981 Jul;27(7):1244-7.
Commercial microcolumns introduced in 1976 by Helena Laboratories ("Hb A2 Quik Column") and by Isolab, Inc. ("Quik-Sep") provide a rapid, simple, accurate method for quantitation of hemoglobin A2 (Hb A2). However, these kits cannot be used for the quantitation of Hb A2 in the presence of slow-moving variants such as Hb S. Recently, Isolab, Inc., produced a new kit ("Quik-Sep Improved Hb A2 Test") for quantitation of both Hb A2 and Hb S. We compared results obtained with the new Isolab kit to results obtained with the original Tris/HCl method for quantitation of Hb A2 and Hb S. Blood was drawn from persons with sickle cell trait (A/S), sickle cell anemia (S/S), sickle cell/beta+ thalassemia (S/beta+ thal) and sickle cell/beta 0 thalassemia (S/beta 0 thal) and percentages of Hb A2 and Hb S were determined by each method. We found no significant differences in Hb A2 percentages by the two methods, and the coefficients of variation were similar. Both methods showed only slight overlap of Hb A2 values from subjects with some form of beta thalassemia and those with A/S or S/S. However, the Tris/HCl method consistently gave values for Hb S that were higher and closer to those expected, suggesting that the Isolab kit does not accurately quantitate Hb S.
1976年由海伦娜实验室推出的商用微柱（“Hb A2快速柱”）以及伊索拉布公司推出的商用微柱（“快速分离柱”）提供了一种快速、简便、准确的血红蛋白A2（Hb A2）定量方法。然而，在存在诸如Hb S等迁移缓慢的变体的情况下，这些试剂盒不能用于Hb A2的定量。最近，伊索拉布公司生产了一种新的试剂盒（“改进的快速分离Hb A2检测试剂盒”）用于Hb A2和Hb S的定量。我们将使用伊索拉布公司新试剂盒获得的结果与使用原始Tris/HCl方法进行Hb A2和Hb S定量获得的结果进行了比较。从具有镰状细胞性状（A/S）、镰状细胞贫血（S/S）、镰状细胞/β+地中海贫血（S/β+地贫）和镰状细胞/β0地中海贫血（S/β0地贫）的个体采集血液，并通过每种方法测定Hb A2和Hb S的百分比。我们发现两种方法测定的Hb A2百分比没有显著差异，变异系数相似。两种方法都显示，患有某种形式β地中海贫血的受试者与患有A/S或S/S的受试者的Hb A2值仅有轻微重叠。然而，Tris/HCl方法得出的Hb S值始终较高且更接近预期值，这表明伊索拉布公司的试剂盒不能准确地定量Hb S。
