Isobe T, Ishioka N, Okuyama T
Eur J Biochem. 1981 Apr;115(3):469-74. doi: 10.1111/j.1432-1033.1981.tb06225.x.
Dodecyl sulfate/urea/polyacrylamide gel electrophoresis of S-100a protein, one of the two major components of the brain-specific S-100 protein, indicated the presence of two different subunits in the protein. These subunits (alpha and beta subunits) were purified from the aminoethylated protein by column chromatography on Sephadex G-75, and the purified subunits were subjected to analyses. The results have shown that S-100a protein is a dimer of alpha and beta subunits, with each subunit having a molecular weight of approximately 10500. Structural comparison of these subunits with the subunit of S-100b protein, the other component of S-100 protein consisting of two identical subunits with known amino acid sequence, has revealed that the beta subunit and the subunit of S-100b protein are identical, so that S-100a protein is related to S-100b protein by sharing one of the subunits as a common structural constituent.
十二烷基硫酸盐/尿素/聚丙烯酰胺凝胶电泳显示，脑特异性S-100蛋白的两个主要成分之一的S-100a蛋白中存在两种不同的亚基。这些亚基（α和β亚基）通过在Sephadex G-75上进行柱色谱从氨乙基化蛋白中纯化出来，并对纯化后的亚基进行分析。结果表明，S-100a蛋白是α和β亚基的二聚体，每个亚基的分子量约为10500。将这些亚基与S-100b蛋白的亚基进行结构比较，S-100b蛋白是S-100蛋白的另一个成分，由两个具有已知氨基酸序列的相同亚基组成，结果发现β亚基与S-100b蛋白的亚基相同，因此S-100a蛋白与S-100b蛋白通过共享一个亚基作为共同的结构成分而相关。
