大鼠纹状体微穿孔组织匀浆中毒蕈碱型胆碱能受体的特性与分布
Properties and distribution of muscarinic cholinergic receptors in rat striatal micropunched tissue homogenates.
作者信息
Takano Y, Uchimura K, Kohjimoto Y, Kamiya H
出版信息
Eur J Pharmacol. 1981 Apr 9;70(4):559-64. doi: 10.1016/0014-2999(81)90367-8.
The properties of muscarinic receptors in rat striatal micropunched tissue homogenates were studied with a binding technique using [3H]quinuclidinyl benzilate (QNB) as ligand. Among the agonists, oxotremorine was the most potent inhibitor (ID50 value, 2.20 X 10(-5) M), followed by pilocarpine, acetylcholine, carbachol and bethanechol. No marked differences were found between the binding properties of QNB in the micropunched tissue homogenates and membrane fractions. Therefore, the detailed distribution of muscarinic receptors in the striatum was examined using the micropunched tissue homogenates. High levels of QNB binding in the striatum were found at dorso-lateral sites and low levels in the center regions of the striatum. The technique presented here for studying small regions of the striatum should be useful in further studies of the function of cholinergic neurons.
采用以[3H]喹核醇基苯甲酸酯（QNB）为配体的结合技术，研究了大鼠纹状体微穿孔组织匀浆中毒蕈碱受体的特性。在激动剂中，氧化震颤素是最有效的抑制剂（半数抑制浓度值，2.20×10⁻⁵ M），其次是毛果芸香碱、乙酰胆碱、卡巴胆碱和氨甲酰甲胆碱。微穿孔组织匀浆和膜组分中QNB的结合特性之间未发现明显差异。因此，使用微穿孔组织匀浆研究了纹状体中毒蕈碱受体的详细分布。在纹状体的背外侧部位发现QNB结合水平较高，而在纹状体的中央区域结合水平较低。本文介绍的用于研究纹状体小区域的技术，在胆碱能神经元功能的进一步研究中应会有用。
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