[3H]-东莨菪碱与大鼠脑内毒蕈碱受体的结合：完整脑片与匀浆结果的比较
[3H]-Quinuclidinyl benzilate binding to muscarinic receptors in rat brain: comparison of results from intact brain slices and homogenates.
作者信息
Gilbert R F, Hanley M R, Iversen L L
出版信息
Br J Pharmacol. 1979 Mar;65(3):451-6. doi: 10.1111/j.1476-5381.1979.tb07850.x.
Abstract
- The binding of [3H]-( +/- )-quinuclidinyl benzilate ([3H]-( +/- )-QNB) to muscarinic sites in rat brain slice and homogenate preparations was compared. 2. Evidence is presented in support of the view that only the (-)-enantiomer of QNB binds with high affinity to muscarinic sites. 3. The Kd value for [3H]-(-)-QNB binding in slices was eight times higher than that measured in homogenates. 4. Similarly, the potencies of various muscarinic ligands as inhibitors of [3H]-(-)-QNB binding were consistently lower in slices than in homogenates. 5. It is proposed that the results may reflect differences in the binding properties of muscarinic receptors in intact tissue slice and homogenate preparations.
摘要
- 比较了[3H] -（±）-喹核醇基苯甲酸酯（[3H] -（±）-QNB）与大鼠脑切片和匀浆制剂中毒蕈碱位点的结合情况。2. 提供的证据支持这样一种观点，即只有QNB的（-）-对映体与毒蕈碱位点具有高亲和力结合。3. 切片中[3H] -（-）-QNB结合的Kd值比匀浆中测得的值高八倍。4. 同样，各种毒蕈碱配体作为[3H] -（-）-QNB结合抑制剂的效力在切片中始终低于匀浆。5. 有人提出，这些结果可能反映了完整组织切片和匀浆制剂中毒蕈碱受体结合特性的差异。
相似文献
1
[3H]-Quinuclidinyl benzilate binding to muscarinic receptors in rat brain: comparison of results from intact brain slices and homogenates.[3H]-东莨菪碱与大鼠脑内毒蕈碱受体的结合：完整脑片与匀浆结果的比较
Br J Pharmacol. 1979 Mar;65(3):451-6. doi: 10.1111/j.1476-5381.1979.tb07850.x.
2
Properties and distribution of muscarinic cholinergic receptors in rat striatal micropunched tissue homogenates.大鼠纹状体微穿孔组织匀浆中毒蕈碱型胆碱能受体的特性与分布
Eur J Pharmacol. 1981 Apr 9;70(4):559-64. doi: 10.1016/0014-2999(81)90367-8.
3
4
Identification of direct competition for, and indirect influences on, striatal muscarinic cholinergic receptors: in vivo [3H]quinuclidinyl benzilate binding in rats.纹状体毒蕈碱胆碱能受体的直接竞争和间接影响的鉴定：大鼠体内[3H]喹核醇基苯甲酸酯结合实验
Brain Res. 1979 Aug 10;171(3):473-80. doi: 10.1016/0006-8993(79)91051-5.
5
Effects of Pro-Leu-Gly-NH2 and cyclo (Leu-Gly) on the binding of 3H-quinuclidinyl benzilate to striatal cholinergic muscarinic receptors.脯氨酸-亮氨酸-甘氨酸-酰胺（Pro-Leu-Gly-NH2）和环（亮氨酸-甘氨酸）（cyclo (Leu-Gly)）对3H-奎宁环基苯甲酸酯与纹状体胆碱能毒蕈碱受体结合的影响。
Peptides. 1986 Jan-Feb;7(1):21-5. doi: 10.1016/0196-9781(86)90055-0.
6
Biochemical indentification of the mammalian muscarinic cholinergic receptor.哺乳动物毒蕈碱型胆碱能受体的生化鉴定
Fed Proc. 1975 Sep;34(10):1915-21.
7
Quinuclidinyl benzilate binding in house fly heads and rat brain.家蝇头部和大鼠脑中的二苯羟乙酸奎宁环酯结合
J Neurochem. 1981 Feb;36(2):454-9. doi: 10.1111/j.1471-4159.1981.tb01614.x.
8
Comparison of [3H]pirenzepine and [3H]quinuclidinylbenzilate binding to muscarinic cholinergic receptors in rat brain.[3H]哌仑西平和[3H]喹核醇基苯甲酸酯与大鼠脑内毒蕈碱胆碱能受体结合的比较
J Pharmacol Exp Ther. 1984 Mar;228(3):648-55.
9
Differential interactions of muscarinic drugs with binding sites of [3H]pirenzepine and [3H]quinuclidinyl benzilate in rat brain tissue.毒蕈碱类药物与大鼠脑组织中[3H]哌仑西平和[3H]喹核醇基苯甲酸酯结合位点的差异相互作用。
Life Sci. 1987 May 18;40(20):1981-7. doi: 10.1016/0024-3205(87)90287-6.
10
Evidence for muscarinic cholinergic receptors in dog portal vein: binding of [3H]quinuclidinyl benzilate.犬门静脉中毒蕈碱型胆碱能受体的证据：[3H]喹核醇基苯甲酸酯的结合
Life Sci. 1983 Apr 11;32(15):1757-63. doi: 10.1016/0024-3205(83)90839-1.
引用本文的文献
1
Acute Lung Injury in Cholinergic-Deficient Mice Supports Anti-Inflammatory Role of α7 Nicotinic Acetylcholine Receptor.胆碱能缺陷小鼠的急性肺损伤支持α7 烟碱型乙酰胆碱受体的抗炎作用。
Int J Mol Sci. 2021 Jul 14;22(14):7552. doi: 10.3390/ijms22147552.
2
The interaction of amine local anaesthetics with muscarinic receptors.胺类局部麻醉药与毒蕈碱受体的相互作用。
Br J Pharmacol. 1980;71(1):327-35. doi: 10.1111/j.1476-5381.1980.tb10943.x.
3
Development of muscarinic cholinergic receptor binding in the visual system of monocularly deprived and dark reared rats.单眼剥夺和黑暗饲养大鼠视觉系统中毒蕈碱型胆碱能受体结合的发育
Neurochem Res. 1982 Sep;7(9):1181-98. doi: 10.1007/BF00964894.
4
The binding characteristics of [3H]-dihydroergocryptine on intact human platelets.[3H]-二氢麦角隐亭在完整人血小板上的结合特性
Br J Pharmacol. 1982 May;76(1):121-30. doi: 10.1111/j.1476-5381.1982.tb09197.x.
5
Muscarinic cholinergic receptor binding in rat mast cells.大鼠肥大细胞中的毒蕈碱型胆碱能受体结合
Agents Actions. 1983 Jun;13(4):327-32. doi: 10.1007/BF01971484.
本文引用的文献
1
Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.大鼠脑中儿茶酚胺的区域研究。I. [3H]去甲肾上腺素、[3H]多巴胺和[3H]多巴在脑不同区域的分布。
J Neurochem. 1966 Aug;13(8):655-69. doi: 10.1111/j.1471-4159.1966.tb09873.x.
2
Absolute configuration of 3-quinuclidinyl benzilate and the behavioral effect in the dog of the optical isomers.3-奎宁环基二苯羟乙酸酯的绝对构型及其光学异构体对犬的行为影响。
J Med Chem. 1972 Sep;15(9):994-5. doi: 10.1021/jm00279a030.
3
In vivo identification of muscarinic cholinergic receptor binding in rat brain.大鼠脑中毒蕈碱型胆碱能受体结合的体内鉴定
Brain Res. 1974 Nov 8;80(1):170-6. doi: 10.1016/0006-8993(74)90738-0.
4
Muscarinic cholinergic binding in rat brain.大鼠脑中的毒蕈碱型胆碱能结合
Proc Natl Acad Sci U S A. 1974 May;71(5):1725-9. doi: 10.1073/pnas.71.5.1725.
5
The binding of (3H)-propylbenzilycholine mustard by longitudinal muscle strips from guinea-pig small intestine.豚鼠小肠纵行肌条对（3H）-丙基苯甲酰胆碱氮芥的结合
Br J Pharmacol. 1974 Jan;50(1):145-51. doi: 10.1111/j.1476-5381.1974.tb09602.x.
6
Ligand binding to muscarinic receptors in intact longitudinal muscle strips from guinea-pig intestine.豚鼠肠道完整纵肌条中配体与毒蕈碱受体的结合
Br J Pharmacol. 1977 Oct;61(2):189-97. doi: 10.1111/j.1476-5381.1977.tb08404.x.
7
Muscarinic acetylcholine receptors interactions: competition binding studies with agonists and antagonists.毒蕈碱型乙酰胆碱受体相互作用：激动剂和拮抗剂的竞争结合研究
Brain Res. 1977 Sep 23;134(1):167-72. doi: 10.1016/0006-8993(77)90936-2.
8
Axotomy causes loss of muscarinic receptors and loss of synaptic contacts in the hypoglossal nucleus.轴突切断术会导致舌下神经核中毒蕈碱受体的丧失和突触联系的丧失。
Nature. 1977 Apr 21;266(5604):734-5. doi: 10.1038/266734a0.
9
Muscarinic receptors: ionic perturbation of the binding properties [proceedings].毒蕈碱受体：结合特性的离子扰动[会议论文集]
Br J Pharmacol. 1977 Mar;59(3):503P.
10
Biochemical studies on muscarinic acetylcholine receptors.毒蕈碱型乙酰胆碱受体的生化研究
J Neurochem. 1976 Jul;27(1):7-16. doi: 10.1111/j.1471-4159.1976.tb01536.x.
Zotero 插件