Effect of cryopreservation on recovery of cells forming colonies in diffusion chambers in mice (CFUD).

The effect of cryopreservation on human and murine hematopoietic precursor cell survival was assessed. A large proportion of CFUC and CFUS was recovered following freezing and thawing. At the same time there was a substantial decrease in the number of cells which form myeloid (CFUDG) and megakaryocytic (CFUDM) colonies in diffusion chambers in mice. We suggest that the CFUDG and CFUDM number should be determined in the autologous bone marrow transplantation setting.