Fabian I, Douer D, Wells J R, Cline M J
Exp Hematol. 1982 Jan;10(1):119-22.
Light density human bone marrow cells were cryopreserved, then thawed and tested for their ability to form myeloid (CFUGM), erythroid (BFUE) and mixed myeloid-erythroid colonies in vitro. Proliferation of mixed hematopoietic colonies was supported by conditioned media from a human T-lymphocyte cell line and erythropoietin. All mixed colonies contained neutrophil and erythroid precursors and approximately 25% had mononuclear phagocytes. Following cryopreservation the bone marrow retained its capacity to form mixed colonies without apparent loss. The recovery of CFUGM and BFUE was 76% and 72% respectively.
低密度人骨髓细胞经冷冻保存，然后解冻，并检测其在体外形成髓系（CFUGM）、红系（BFUE）以及混合髓系-红系集落的能力。人T淋巴细胞系的条件培养基和促红细胞生成素支持混合造血集落的增殖。所有混合集落均含有中性粒细胞和红系前体细胞，约25%含有单核吞噬细胞。冷冻保存后，骨髓保留了形成混合集落的能力，且无明显损失。CFUGM和BFUE的回收率分别为76%和72%。
