Glaves D
Int J Cancer. 1980 Jul 15;26(1):115-22. doi: 10.1002/ijc.2910260118.
The lung retention patterns of B16 melanoma cells were determined after intravenous injection of [125l]dUrd-labelled tumor cells into B16 melanomabearing mice. Experiments were performed with mice undergoing acute or chronic reactions to bacterial endotoxin or zymosan, both of which were shown to modify the activity of the reticuloendothelial system as assessed by carbon clearance assays. On the one hand, the release of arrested melanoma cells from the lungs was retarded in mice with both endotoxin-and zymosan- induced acute inflammation. There was a parallel increase in the numbers of pulmonary tumor nodules which developed after injection of non-radiolabelled melanoma cells into similarly treated groups of mice. On the other hand, fewer tumor cells were retained in the lungs of mice undergoing chronic responses to endotoxin, and fewer pulmonary tumor nodules subsequently arose after injection of unlabelled cells. However, in mice pre-treated with zymosan, retention of melanoma cells was not different from that in controls and greatly increased numbers of pulmonary nodules grew in zymosan pre-treated mice receiving non-radiolabelled cells. These experiments were discussed in terms of the contribution of the reticuloendothelial system to the release of arrested cancer cells from the pulmonary vasculature.
将[¹²⁵I]脱氧尿苷标记的肿瘤细胞静脉注射到携带B16黑色素瘤的小鼠体内后，测定了B16黑色素瘤细胞在肺部的滞留模式。实验在对细菌内毒素或酵母聚糖产生急性或慢性反应的小鼠身上进行，通过碳清除试验评估，这两种物质均显示会改变网状内皮系统的活性。一方面，在内毒素和酵母聚糖诱导的急性炎症小鼠中，肺部滞留的黑色素瘤细胞的释放受到延迟。在将未标记的黑色素瘤细胞注射到经过类似处理的小鼠组后，肺部肿瘤结节的数量相应增加。另一方面，在内毒素慢性反应的小鼠肺部，滞留的肿瘤细胞较少，注射未标记细胞后随后出现的肺部肿瘤结节也较少。然而，在酵母聚糖预处理的小鼠中，黑色素瘤细胞的滞留与对照组无异，在接受未标记细胞的酵母聚糖预处理小鼠中，肺部结节的数量大幅增加。根据网状内皮系统对肺部血管中滞留癌细胞释放的作用对这些实验进行了讨论。
