Glaves D, Huben R P, Weiss L
Department of Experimental Pathology, Rosewell Park Memorial Institute, Buffalo, NY 14263.
Br J Cancer. 1988 Jan;57(1):32-5. doi: 10.1038/bjc.1988.4.
Estimates were made of the rates at which cancer cells were released directly into the renal vein in patients undergoing radical nephrectomy for primary renal cancer. Cancer cells were counted in blood samples taken from the renal vein using a density gradient centrifugation procedure, and identified using immunocytochemical techniques, on the basis of their cytoskeletal intermediate filament proteins. Cancer cells were released as single cells and multicell emboli in 8/10 patients, in numbers varying widely between 14-7509 emboli ml-1 of blood. Despite a calculated median input into the metastatic process of 3.7 x 10(7) cancer cells per day for at least 180 days, only 3/10 patients had extraperitoneal metastases prior to surgery and only 1 of the remaining disease-free patients subsequently developed distant metastases over a maximum 35 month period. These results are discussed in terms of primary tumour kinetics and metastatic inefficiency.
对因原发性肾癌接受根治性肾切除术患者的癌细胞直接释放到肾静脉中的速率进行了估计。使用密度梯度离心法对从肾静脉采集的血样中的癌细胞进行计数，并根据其细胞骨架中间丝蛋白，采用免疫细胞化学技术进行鉴定。在10例患者中有8例，癌细胞以单细胞和多细胞栓子的形式释放，每毫升血液中栓子数量在14 - 7509之间广泛变化。尽管计算得出，在至少180天的时间里，每天进入转移过程的癌细胞中位数为3.7×10⁷个，但在手术前只有3/10的患者有腹膜外转移，在最长35个月的时间里，其余无病患者中只有1例随后发生远处转移。根据原发性肿瘤动力学和转移效率低下对这些结果进行了讨论。
