Kramer R H, Gonzalez R, Nicolson G L
Int J Cancer. 1980 Nov 15;26(5):639-45. doi: 10.1002/ijc.2910260516.
Two metastatic cell lines, mouse B16-Fl melanoma and human Hs939 melanoma, were examined for their abilities to adhere to confluent vascular endothelial cell monolayers and to the underlying endothelial extracellular matrix. Tumor cells attacked slowly to the endothelial cell monolayers while they adhered rapidly to isolated extracellular matrix. When analyzed by polyacrylamide gel electrophoresis in sodium dodecylsulfate solutions, the extracellular matrix was shown to be primarily composed of a protein of identical migration and molecular weight to fibronectin. Tumor-cell adhesion to fibronectin-coated polyvinyl surfaces mimicked the rapid rate of attachment of tumor cells to extracellular matrix, and tumor cells adherent to either extracellular matrix or fibronectin-coated polyvinyl dishes adopted an unusual, highly spread and flattened morphology with numerous small projections. These results suggest that fibronectin associated with the endothelial basement membrane may be, in part, responsible for establishing an adhesive gradient that could be important in malignant cell extravasation.
对两种转移性细胞系，即小鼠B16-Fl黑色素瘤细胞系和人Hs939黑色素瘤细胞系，检测了它们黏附于汇合的血管内皮细胞单层以及下方内皮细胞外基质的能力。肿瘤细胞缓慢侵袭内皮细胞单层，而它们能迅速黏附于分离的细胞外基质。当在十二烷基硫酸钠溶液中通过聚丙烯酰胺凝胶电泳进行分析时，细胞外基质显示主要由一种迁移率和分子量与纤连蛋白相同的蛋白质组成。肿瘤细胞对纤连蛋白包被的聚乙烯表面的黏附模拟了肿瘤细胞对细胞外基质的快速黏附速率，并且黏附于细胞外基质或纤连蛋白包被的聚乙烯培养皿的肿瘤细胞呈现出一种异常的、高度铺展和平坦的形态，带有许多小突起。这些结果表明，与内皮基底膜相关的纤连蛋白可能部分负责建立一种黏附梯度，这在恶性细胞外渗中可能很重要。
