Vollmers H P, Imhof B A, Braun S, Waller C A, Schirrmacher V, Birchmeier W
FEBS Lett. 1984 Jun 25;172(1):17-20. doi: 10.1016/0014-5793(84)80863-7.
Cellular adhesion is important during metastasis, as metastatic cells must escape from the primary site into lymph and blood systems, there to adhere specifically to sites in distant organs. We have recently selected monoclonal antibodies which prevent adherence of B16 mouse melanoma cells to tissue culture dishes, and also markedly reduce experimental lung metastasis in mice when injected before or with the tumor cells. Here, we investigated which step in the metastatic process may be affected by the antibodies. The possible inhibitory effect of antibody on tumour cell adherence to vascular endothelial monolayers and to purified components of the underlying extracellular matrix - fibronectin, laminin and collagen type IV - was studied using in vitro assays. We found that the antibodies significantly blocked attachment to laminin, suggesting that specific basement membrane components play an important role in attracting or otherwise modifying the behaviour of metastatic tumour cells.
细胞黏附在转移过程中很重要，因为转移细胞必须从原发部位逃逸进入淋巴和血液系统，并在那里特异性地黏附到远处器官的位点。我们最近筛选出了单克隆抗体，这些抗体可阻止B16小鼠黑色素瘤细胞黏附于组织培养皿，并且在与肿瘤细胞同时注射或在其之前注射时，还能显著减少小鼠的实验性肺转移。在此，我们研究了转移过程中的哪一步可能受到这些抗体的影响。使用体外试验研究了抗体对肿瘤细胞黏附于血管内皮单层以及对下层细胞外基质的纯化成分——纤连蛋白、层粘连蛋白和IV型胶原——的可能抑制作用。我们发现这些抗体显著阻断了对层粘连蛋白的附着，表明特定的基底膜成分在吸引或改变转移肿瘤细胞的行为方面发挥着重要作用。
