Jaroensanti J, Panijpan B
Int J Vitam Nutr Res. 1981;51(1):34-8.
Bisulphite/sulphite cleaves thiamine and its biological phosphorylated derivatives into the same pyrimidine moiety and their respective thiazole moiety with pseudo-first order kinetic constants following the order of thiamine greater than thiamine monophosphate greater than thiamine diphosphate greater than thiamine triphosphate over the pH range of 3.9 to 7.0. The activation energy was 14.8 kcal/mol for all four thiamines at pH 5.2 and over the temperature range of 25 to 65 degrees C. The rate differences may be rationalized in terms of differences in the pH-dependent electronic, charge and conformation distributions of the various thiamine species.
亚硫酸氢盐/亚硫酸盐在pH值3.9至7.0的范围内，将硫胺素及其生物磷酸化衍生物裂解为相同的嘧啶部分和各自的噻唑部分，其准一级动力学常数遵循硫胺素大于硫胺素单磷酸酯大于硫胺素二磷酸酯大于硫胺素三磷酸酯的顺序。在pH值5.2以及25至65摄氏度的温度范围内，所有四种硫胺素的活化能均为14.8千卡/摩尔。速率差异可以根据各种硫胺素物种在pH值依赖性电子、电荷和构象分布上的差异来解释。
