Marcus D C, Marcus N Y, Thalmann R
Hear Res. 1981 May;4(2):149-60. doi: 10.1016/0378-5955(81)90002-2.
Perfusion of the perilymphatic space of guinea pig cochleae with K-free medium leads to a gradual decline of the endocochlear potential (EP) over 30-50 min to a negative value (mean: -12 mV). The input resistance of scala media does not decrease during this time. The ATP and K content of the stria vascularis are reduced by similar amounts (26 and 34%, respectively) during this period. Perfusion of 1 mM ouabain produces a different pattern of response: strial ATP remains normal while strial K content is strongly reduced (by 77%). Strial Na rises in a complementary way to the K loss. These results demonstrate that a reduction of the K concentration of the perilymph leads to an inhibition of the generator of the positive component of the EP rather than to a general increase of cochlear duct membrane conductance. In addition, they suggest, in concert with other considerations (such as the slower rate of decline of the EP during K-free vascular perfusion (Wada, J., Kambayashi, J., Marcus, D.C. and Thalmann, R (1979): Arch. Otorhinolaryngol. 225, 79-81)), that the mode of action may be different from that of ouabain. In spite of the lack of teleological support, we offer the hypothesis that the strial generator of the EP may primarily utilize K from perilymph and that vascular K may not have access to the generator.
用无钾培养基灌注豚鼠耳蜗的外淋巴间隙会导致内淋巴电位（EP）在30 - 50分钟内逐渐下降至负值（平均：-12 mV）。在此期间，中阶的输入电阻并未降低。在此期间，血管纹的ATP和钾含量分别减少了相似的量（分别为26%和34%）。灌注1 mM哇巴因会产生不同的反应模式：血管纹ATP保持正常，而血管纹钾含量大幅降低（降低77%）。血管纹钠以与钾流失互补的方式升高。这些结果表明，外淋巴钾浓度的降低会导致EP正性成分的产生器受到抑制，而不是耳蜗管膜电导普遍增加。此外，与其他因素（如无钾血管灌注期间EP下降速度较慢（和田，J.，神林，J.，马库斯，D.C.和塔尔曼，R（1979年）：耳鼻咽喉科文献 225，79 - 81））一致，它们表明作用方式可能与哇巴因不同。尽管缺乏目的论支持，我们提出假说，即EP的血管纹产生器可能主要利用外淋巴中的钾，而血管中的钾可能无法进入产生器。
