Joe F L, Roseboro E L, Fazio T
J Assoc Off Anal Chem. 1981 May;64(3):641-6.
A high pressure liquid chromatographic (HPLC) method has been developed for the determination of some polynuclear aromatic hydrocarbons (PAHs) in beer. The PAHs are extracted into isooctane from a beer sample to which sodium hydroxide, ethanol, and acetone have been added. The isooctane extract is washed with acid and then base, and is purified by chromatography through deactivated neutral alumina. The eluate from the column is concentrated to dryness and the resulting residue is dissolved in methanol-acetonitrile (1 + 1) and subjected to reverse phase HPLC analysis. Both ultraviolet (UV) and fluorescence detectors are used to monitor the HPLC column effluent. Recovery of 4 PAHs, benz(a)anthracene, benzo(a)pyrene, benzo(g,h,i)perylene, and dibenzo(a,i)pyrene, ranged from 77 to 108% by UV measurement and 73 to 97% by fluorescence measurement.
已开发出一种高压液相色谱（HPLC）方法，用于测定啤酒中的某些多环芳烃（PAH）。将氢氧化钠、乙醇和丙酮添加到啤酒样品中，然后将PAH从啤酒样品中萃取到异辛烷中。异辛烷萃取液先用酸洗涤，再用碱洗涤，然后通过失活的中性氧化铝柱色谱进行纯化。柱洗脱液浓缩至干，所得残渣溶于甲醇 - 乙腈（1 + 1）中，进行反相HPLC分析。使用紫外（UV）和荧光检测器监测HPLC柱流出物。通过UV测量，4种PAH，即苯并（a）蒽、苯并（a）芘、苯并（g，h，i）苝和二苯并（a，i）芘的回收率在77%至108%之间；通过荧光测量，回收率在73%至97%之间。
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