Hanus J P, Guerrero H, Biehl E R, Kenner C T
J Assoc Off Anal Chem. 1979 Jan;62(1):29-35.
A high pressure liquid chromatographic (HPLC) procedure is described for determining 13 polynuclear aromatic hydrocarbon (PNA) compounds in oysters at the 2 ppb level. These compounds are extracted from shellfish with acetonitrile and partitioned into petroleum ether; the petroleum ether is removed and the residue is saponified. The aromatic compounds are isolated by passing the saponifeid residue through silica gel and further purified and fractionated by muStyragel gel permeation chromatography. The in-ividual PNAs are then quantitatively determined by using a reverse phase HPLC column coupled to fluorescence, spectrophotometric, and 254 nm absorbance detectors in series. Recoveries from spiked samples generally were greater than 80%.
描述了一种高压液相色谱（HPLC）方法，用于测定牡蛎中浓度为2 ppb的13种多环芳烃（PNA）化合物。这些化合物用乙腈从贝类中提取出来，然后分配到石油醚中；去除石油醚，将残渣皂化。通过使皂化后的残渣通过硅胶来分离芳香族化合物，再通过μStyragel凝胶渗透色谱法进一步纯化和分馏。然后使用串联的反相HPLC柱与荧光、分光光度和254 nm吸光度检测器对各个PNA进行定量测定。加标样品的回收率一般大于80%。
