Joe F L, Salemme J, Fazio T
J Assoc Off Anal Chem. 1984 Nov-Dec;67(6):1076-82.
A liquid chromatographic (LC) method was developed and applied to the determination of polynuclear aromatic hydrocarbons (PAHs) in a variety of smoked, market basket commodities. The PAHs are extracted with 1,1,2-trichloro-1,2,2-trifluoroethane (Freon 113) from alcoholic KOH digests of the commodities. The extracts are purified by column chromatography through a deactivated silica gel/alumina column and by liquid-liquid partitioning between dimethyl sulfoxide and cyclohexane before separation of the PAHs by LC. Both fluorescence and UV detectors are used to monitor the LC column effluent to detect nanogram quantities of PAHs. Trace levels of carcinogenic and non-carcinogenic PAHs were found in all samples analyzed, although generally at less than 1 ppb levels.
开发了一种液相色谱（LC）方法，并将其应用于测定各种烟熏食品及市场采购篮商品中的多环芳烃（PAH）。用1,1,2-三氯-1,2,2-三氟乙烷（氟利昂113）从商品的乙醇氢氧化钾消化液中提取多环芳烃。提取物先通过失活硅胶/氧化铝柱进行柱色谱纯化，然后在通过液相色谱分离多环芳烃之前，在二甲基亚砜和环己烷之间进行液-液分配。荧光检测器和紫外检测器均用于监测液相色谱柱流出物，以检测纳克级的多环芳烃。在所分析的所有样品中均发现了痕量水平的致癌和非致癌多环芳烃，不过一般含量低于1 ppb。
