用于检测肠道寄生虫的福尔马林-乙醚沉淀法、福尔马林-乙酸乙酯沉淀法和硫酸锌浮选法的比较
Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques for detection of intestinal parasites.
作者信息
Truant A L, Elliott S H, Kelly M T, Smith J H
出版信息
J Clin Microbiol. 1981 May;13(5):882-4. doi: 10.1128/jcm.13.5.882-884.1981.
Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were encountered. The Formalin-ether and Formalin-ethyl acetate sedimentation procedures gave identical results for the detection of cysts, ova, and larvae, and these methods offered an advantage over the flotation procedure for the detection of selected ova. However, the zinc sulfate procedure was more effective for the detection of protozoan cysts, Hymenolepis nana, and hookworm eggs. The results indicate that the Formalin-ethyl acetate procedure provides a suitable alternative to the Formalin-ether method, and they demonstrate the value of using both flotation and sedimentation procedures in the analysis of fecal specimens for parasites.
使用250多个临床寄生虫学标本对福尔马林-乙醚沉淀法、福尔马林-乙酸乙酯沉淀法和硫酸锌浮选法进行了比较。鉴定出50个阳性标本，发现了多种寄生虫，包括阿米巴原虫、鞭毛虫、绦虫、线虫和吸虫。福尔马林-乙醚和福尔马林-乙酸乙酯沉淀法在检测囊肿、虫卵和幼虫方面结果相同，并且这些方法在检测特定虫卵方面比浮选法更具优势。然而，硫酸锌法在检测原生动物囊肿、微小膜壳绦虫和钩虫卵方面更有效。结果表明，福尔马林-乙酸乙酯法是福尔马林-乙醚法的合适替代方法，并且它们证明了在粪便标本寄生虫分析中同时使用浮选法和沉淀法的价值。
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