一种使用蒽酮试剂同时测定生物样品中总碳水化合物和甘油的方法。
A method for the simultaneous determination of total carbohydrate and glycerol in biological samples with the anthrone reagent.
作者信息
Pons A, Roca P, Aguiló C, Garcia F J, Alemany M, Palou A
出版信息
J Biochem Biophys Methods. 1981 Mar;4(3-4):227-31. doi: 10.1016/0165-022x(81)90060-9.
A method for quantitative estimation of glycerol and total carbohydrate in biological samples is described. The samples, deproteinized with cold acetone or trichloroacetic acid, were treated in the cold with 10 vols. of 0.75% (w/w) anthrone in 84% (w/w) sulphuric acid, and then heated 10 min at 100 degrees C. Absorbance at 590 nm was used for evaluation of total carbohydrate content in the sample. The absorbance at 510 nm was used for the combined carbohydrate and glycerol estimation. This latter observation leads to the determination of the glycerol content of the sample because the carbohydrate interference is known from the data obtained at 590 nm. Uses of this method to determine glycerol and carbohydrate content in lipids of chicken egg-yolk samples are presented.
本文描述了一种定量估算生物样品中甘油和总碳水化合物的方法。用冷丙酮或三氯乙酸进行脱蛋白处理后的样品，在低温下用10倍体积的0.75%（w/w）蒽酮于84%（w/w）硫酸中处理，然后在100℃加热10分钟。利用590nm处的吸光度评估样品中的总碳水化合物含量。利用510nm处的吸光度估算碳水化合物和甘油的总量。由于从590nm处获得的数据已知碳水化合物的干扰情况，因此可通过后一种观察结果来测定样品中的甘油含量。本文还介绍了该方法在测定鸡卵黄样品脂质中甘油和碳水化合物含量方面的应用。
Zotero 插件