Gao Guanzhen, He Chuanqi, Wang Huiqin, Guo Jingke, Ke Lijing, Zhou Jianwu, Chong Pik Han, Rao Pingfan
Food Nutrition Science Centre, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310012, China.
Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
Nanomaterials (Basel). 2021 Dec 23;12(1):30. doi: 10.3390/nano12010030.
It has been revealed that numerous nanoparticles are formed during the boiling preparation of traditional Chinese medical decoctions and culinary soups. They may possess physiological effects different from those of constituent components and are worth paying attention to but are barely noticed and investigated as of yet. In this study, six groups of nanoparticles, whose size ranged from 57 to 300 nm, were successfully isolated from the decoction of Fort. root, according to their particle size by the means of size-exclusive chromatography. All of the obtained nanoparticles have a high content of polysaccharides, which distinguishes them from the disclosed BLG protein nanoparticles. They also have high similarities in other compositions, surface charge, and stimuli responses. However, four out of these six nanoparticles (F2, F3, F4, and F5) exhibited significant antiviral activity against influenza virus H1N1, and their antiviral activities and cytotoxicity towards MDCK cells varied with their sizes. It suggested that the antiviral efficacy of BLG decoction could also be from its nanoparticles besides its well-known antiviral phytochemicals. It also implied that the biological effects of these polysaccharide nanoparticles, including cytotoxicity and antiviral activity, may be correlative with the physicochemical properties, especially the particle size.
据揭示，在传统中药汤剂和烹饪汤品的熬制过程中会形成大量纳米颗粒。它们可能具有与组成成分不同的生理效应，值得关注，但迄今为止几乎未被注意和研究。在本研究中，通过尺寸排阻色谱法，根据颗粒大小，从Fort. root汤剂中成功分离出六组尺寸范围为57至300纳米的纳米颗粒。所有获得的纳米颗粒都含有高含量的多糖，这使它们与已公开的BLG蛋白纳米颗粒有所区别。它们在其他成分、表面电荷和刺激反应方面也有高度相似性。然而，这六个纳米颗粒中的四个（F2、F3、F4和F5）对甲型流感病毒H1N1表现出显著的抗病毒活性，并且它们对MDCK细胞的抗病毒活性和细胞毒性随其大小而变化。这表明BLG汤剂的抗病毒功效除了其众所周知的抗病毒植物化学物质外，也可能来自其纳米颗粒。这也意味着这些多糖纳米颗粒的生物学效应，包括细胞毒性和抗病毒活性，可能与物理化学性质相关，尤其是颗粒大小。
