β2-微球蛋白与兔和豚鼠主要组织相容性复合体编码的同种抗原之间的关系。

Relationships between beta2-microglobulin and alloantigens coded for by the major histocompatibility complexes of the rabbit and the guinea pig.

作者信息

Björck L, Cigén R, Berggård B, Löw B, Berggård I

出版信息

Scand J Immunol. 1977;6(10):1063-9. doi: 10.1111/j.1365-3083.1977.tb00342.x.

DOI:10.1111/j.1365-3083.1977.tb00342.x

Abstract

Treatment of rabbit and guinea pig lymphocytes with Fab' fragments of anti-beta2-microglobulin completely inhibited the cytotoxic effects of alloantisera to RLA or GPLA antigens, respectively. Aggregation of beta2-microglobulin on the lymphocyte surface by successive incubations with goat anti-beta2-microglobulin on the lymphocyte surface by successive incubations with goat anti-beta2-microglobulin and F(ab')2 fragments of rabbit anti-goat IgG also made rabbit lymphocytes resistant to lysis by anti-RLA, and guinea pig lymphocytes resistant to lysis by anti-RLA, and guinea pig lymphocytes resistant to lysis by anti-GPLA. The two kinds of pretreatment of guinea pig lymphocytes did not affect the cytotoxicity of antisera directed against guinea pig Ia antigens. These results in conjunction with previous findings in the mouse and in man suggest that beta2-microglobulin on the lymphocyte surface in mammals is generally associated with major serologically defined histocompatibility antigens but not with I-region-associated antigens.

摘要

用抗β2-微球蛋白的Fab'片段处理兔和豚鼠淋巴细胞，分别完全抑制了同种抗血清对兔淋巴细胞同种抗原（RLA）或豚鼠淋巴细胞同种抗原（GPLA）的细胞毒性作用。通过先后用山羊抗β2-微球蛋白以及兔抗山羊IgG的F(ab')2片段与淋巴细胞表面进行孵育，使β2-微球蛋白在淋巴细胞表面聚集，这也使得兔淋巴细胞对抗RLA裂解具有抗性，豚鼠淋巴细胞对抗GPLA裂解具有抗性。豚鼠淋巴细胞的这两种预处理方式均未影响针对豚鼠Ia抗原的抗血清的细胞毒性。这些结果与先前在小鼠和人类中的研究结果相结合，表明哺乳动物淋巴细胞表面的β2-微球蛋白通常与主要血清学定义的组织相容性抗原相关，但与I区相关抗原无关。