Kronvall G, Myhre E B, Björck L, Berggård I
Infect Immun. 1978 Oct;22(1):136-42. doi: 10.1128/iai.22.1.136-142.1978.
A novel mammalian-microbial "short circuit" has been demonstrated between aggregated human beta2-microglobulin and group A, C, and G streptococci. Bacteria belonging to nine gram-positive and three gram-negative species were tested for binding of radiolabeled beta2-microglobulin. All 10 individual strains of group A streptococci showed a high degree of reactivity with aggregated human beta2-microglobulin. Among 27 group C and 28 group G streptococci, 9 and 6 strains, respectively, were highly reactive, whereas the remaining strains showed a lower, but definite level of beta2-microglobulin binding. Of 11 group B streptococci, 4 were slightly positive. All strains among the other eight species were completely negative. Simultaneous testing of A, C, and G streptococci for immunoglobulin binding showed a lack of correlation between type II and III Fc reactivity and beta2-microglobulin binding. There was no inhibition of uptake of aggregated beta2-microglobulin to reactive strains when excess amounts of human immunoglobulin were added. The beta2-microglobulin-binding surface structure was found to be markedly sensitive to trypsin digestion. The relative trypsin resistance of the immunoglobulin-binding protein in the digestion experiments further demonstrated the dissociation between these two reactivities.
已证实聚集的人β2-微球蛋白与A、C和G组链球菌之间存在一种新型的哺乳动物-微生物“短路”。对属于9种革兰氏阳性菌和3种革兰氏阴性菌的细菌进行了放射性标记的β2-微球蛋白结合测试。所有10株A组链球菌个体均显示出与聚集的人β2-微球蛋白高度反应。在27株C组和28株G组链球菌中，分别有9株和6株高度反应，而其余菌株显示出较低但确定的β2-微球蛋白结合水平。11株B组链球菌中，4株呈弱阳性。其他8个菌种的所有菌株均完全阴性。对A、C和G组链球菌进行免疫球蛋白结合的同时测试表明，II型和III型Fc反应性与β2-微球蛋白结合之间缺乏相关性。当加入过量的人免疫球蛋白时，聚集的β2-微球蛋白对反应性菌株的摄取没有受到抑制。发现β2-微球蛋白结合表面结构对胰蛋白酶消化明显敏感。消化实验中免疫球蛋白结合蛋白相对抗胰蛋白酶的特性进一步证明了这两种反应性之间的分离。
