抗去唾液酸GM1的体内效应。I. 裸鼠自然杀伤细胞活性降低及移植瘤生长增强

In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice.

作者信息

Habu S, Fukui H, Shimamura K, Kasai M, Nagai Y, Okumura K, Tamaoki N

出版信息

J Immunol. 1981 Jul;127(1):34-8.

DOI:

Abstract

Intravenous injection of anti-asialo GM1, which has been shown to eliminate natural killer (NK) activity in vitro in the presence of complement, completely abolished NK activity against lymphoma cell line (YAC-1) in spleen cells from athymic nude mice as well as from conventional mice. An immunofluorescence study revealed a decreased number of asialo GM1 positive cells in the spleens of mice injected with anti-asialo GM1 than in those of mice injected with normal rabbit serum. In the nude mice with reduced NK activity, incidence of tumor take and the growth were enhanced when syngeneic (RL male-1), and allogeneic (YAC-1) tumors and human tumors were transplanted subcutaneously. These results strongly suggest that NK cells play an important role in transplanted-tumor growth in vivo.

摘要

静脉注射抗唾液酸GM1已被证明在补体存在的情况下可在体外消除自然杀伤（NK）活性，它完全消除了无胸腺裸鼠以及常规小鼠脾细胞中针对淋巴瘤细胞系（YAC-1）的NK活性。免疫荧光研究显示，注射抗唾液酸GM1的小鼠脾脏中唾液酸GM1阳性细胞的数量比注射正常兔血清的小鼠少。在NK活性降低的裸鼠中，当同基因（RL male-1）、异基因（YAC-1）肿瘤和人类肿瘤皮下移植时，肿瘤接种率和生长均增强。这些结果强烈表明NK细胞在体内移植肿瘤生长中起重要作用。