Cox C P, Wardlow M L, Jorgensen R, Farr R S
J Immunol. 1981 Jul;127(1):46-50.
This report describes PAF activity in normal human mixed saliva from each of 24 randomly selected donors. The human salivary PAF (HS-PAF) was similar to rabbit basophil PAF (acetyl glyceryl ether phosphorylcholine or AGEPC) with respect to the following characteristics: 1. HS-PAF co-chromatographed with the standard rabbit basophil AGEPC and with synthetic AGEPC. 2. HS-PAF and AGEPC were unaffected by hirudin, indomethacin, and creatine phosphate/creatine phosphokinase, the respective inhibitors of platelet activation induced by thrombin, arachidonic acid, and ADP. 3. HS-PAF and AGEPC were inactivated by a 5-min incubation with ALF, the acid-labile factor in normal human serum that rapidly destroys AGEPC. 4. HS-PAF was demonstrated to be functionally similar to AGEPC by cross-desensitization experiments. In the absence of Ca++, HS-PAF and AGEPC cross-desensitized washed rabbit platelets to subsequent stimulation by either HS-PAF or AGEPC after recalcification. 5. HS-PAF was demonstrated to be structurally similar to AGEPC by several simple chemical tests for functional groups.
本报告描述了从24名随机选择的供体中获取的正常人混合唾液中的血小板活化因子（PAF）活性。人唾液PAF（HS-PAF）在以下特性方面与兔嗜碱性粒细胞PAF（乙酰甘油醚磷酸胆碱或AGEPC）相似：1. HS-PAF与标准兔嗜碱性粒细胞AGEPC以及合成AGEPC共色谱。2. HS-PAF和AGEPC不受水蛭素、消炎痛以及磷酸肌酸/磷酸肌酸激酶的影响，这些分别是由凝血酶、花生四烯酸和ADP诱导的血小板活化的抑制剂。3. HS-PAF和AGEPC与正常人血清中的酸不稳定因子ALF孵育5分钟后失活，ALF能迅速破坏AGEPC。4. 通过交叉脱敏实验证明HS-PAF在功能上与AGEPC相似。在无Ca++的情况下，HS-PAF和AGEPC使洗涤后的兔血小板对再钙化后随后由HS-PAF或AGEPC引起的刺激产生交叉脱敏。5. 通过对官能团的几种简单化学测试证明HS-PAF在结构上与AGEPC相似。
