Ozasa H, Tominaga T, Nishimura T, Takeda T
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Oct;32(10):1567-74.
Fibroblasts grown from abdominal and vulvar skin from normal women were tested for their dihydrotestosterone binding activity. Fibroblasts from either source had 8S cytoplasmic dihydrotestosterone receptors as confirmed by sucrose gradient analysis and the concentration of dihydrotestosterone binding sites and the apparent dissociation constant were 21.6 +/- 1.1 fmoles/mg protein and 0.50 +/- 0.028 nM, respectively, for fibroblasts from abdominal skin and 35.9 +/- 1.7 fmoles/mg protein and 0.24 +/- 0.015 nM, respectively, for fibroblasts from vulvar skin. The dihydrotestosterone binding activity was greater in fibroblasts from vulvar skin than from abdominal skin (P less than 0.01). Fibroblasts formed dense monolayers were treated with various concentrations of dihydrotestosterone (10, 100, 1,000 ng/ml) for six days and were assessed for hormone effect. The production of proteins was elevated in fibroblasts from either source with a slightly greater hormonal response occurring in fibroblasts from vulvar skin than from abdominal skin. Collagen production was elevated significantly in fibroblasts from vulvar skin but only marginally in fibroblasts from abdominal skin. The synthesis of DNA was not affected in fibroblasts from either source. These results than response to dihydrotestosterone was greater in fibroblasts with a higher hormone binding activity may reflect the hormone effect being mediated through dihydrotestosterone receptors.
对从正常女性腹部和外阴皮肤培养的成纤维细胞进行了二氢睾酮结合活性测试。通过蔗糖梯度分析证实，来自这两种来源的成纤维细胞均具有8S细胞质二氢睾酮受体，腹部皮肤成纤维细胞的二氢睾酮结合位点浓度和表观解离常数分别为21.6±1.1飞摩尔/毫克蛋白质和0.50±0.028纳摩尔，外阴皮肤成纤维细胞分别为35.9±1.7飞摩尔/毫克蛋白质和0.24±0.015纳摩尔。外阴皮肤成纤维细胞的二氢睾酮结合活性高于腹部皮肤成纤维细胞（P<0.01）。将形成致密单层的成纤维细胞用不同浓度的二氢睾酮（10、100、1000纳克/毫升）处理6天，并评估激素效应。两种来源的成纤维细胞中蛋白质的产生均升高，外阴皮肤成纤维细胞的激素反应略高于腹部皮肤成纤维细胞。外阴皮肤成纤维细胞中胶原蛋白的产生显著升高，但腹部皮肤成纤维细胞中仅略有升高。两种来源的成纤维细胞中DNA的合成均未受影响。这些结果表明，激素结合活性较高的成纤维细胞对二氢睾酮的反应更大，这可能反映了激素效应是通过二氢睾酮受体介导的。
