Yamada Y
Nihon Sanka Fujinka Gakkai Zasshi. 1980 Nov;32(11):1820-8.
There have been many studies on the cellular immune responses against human tumors using in vitro cytotoxicity tests, but information concerning specific cell-mediated immunity against human autologous tumors is scant because of technological difficulties. In this paper, peripheral blood lymphocytes from patients with cervical cancer were tested by a microcytotoxicity assay for tumor specific immunity against autologous target cells of the primary cultures. Blocking serum activity was also examined. The following results were obtained: 1) In 37 cases of cervical cancer, mean cytotoxicity was 40.5% for patients in stage I, 28.4% in stage II amd 14.5% in stage III. 2) Mean cytotoxicity was significantly decreased in patients with regional lymph node metastasis (15.3%) compared with that in patients without regional lymph node metastasis (35.7%). 3) Mean serum blocking activity on cell-mediated cytotoxicity was increased in patients with regional lymph node metastasis (18.1%) compared with that in patients without regional lymph node metastasis (7.5%). 4) A significant correlation was found between specific and non-specific cell-mediated immunity (skin reactivity to DNCB and lymphocyte response to PHA). From the above results, it may be deduced that both specific and non-specific cell-mediated immunity decrease while serum blocking activity increases as the autologous tumor grows.
已经有许多关于使用体外细胞毒性试验针对人类肿瘤的细胞免疫反应的研究，但由于技术困难，关于针对人类自体肿瘤的特异性细胞介导免疫的信息很少。在本文中，通过微细胞毒性试验检测了宫颈癌患者外周血淋巴细胞对原代培养的自体靶细胞的肿瘤特异性免疫。还检测了阻断血清活性。获得了以下结果：1）在37例宫颈癌患者中，I期患者的平均细胞毒性为40.5%，II期为28.4%，III期为14.5%。2）与无区域淋巴结转移的患者（35.7%）相比，有区域淋巴结转移的患者（15.3%）的平均细胞毒性显著降低。3）与无区域淋巴结转移的患者（7.5%）相比，有区域淋巴结转移的患者（18.1%）对细胞介导细胞毒性的平均血清阻断活性增加。4）发现特异性和非特异性细胞介导免疫之间存在显著相关性（对二硝基氯苯的皮肤反应性和对PHA的淋巴细胞反应）。从上述结果可以推断，随着自体肿瘤的生长，特异性和非特异性细胞介导免疫均降低，而血清阻断活性增加。
