Watanabe K
Nihon Sanka Fujinka Gakkai Zasshi. 1980 May;32(5):635-43.
By means of dextran-coated charcoal assay, the capacity of various endometrial cytosol preparation for specific binding of 3H-progesterone was determined. After sucrose gradient ultracentrifugation progesterone-binding components with sedimentation coefficients of 8S and 5S were found. Especially, 8S binding component tended to be saturated and highly specific for progesterone. And its dissociation constant and studies of competition with various unlabeled steroids revealed no significant difference between normal and abnormal endometrium. This 8S receptor in normal endometrium with regular menstrual cycle revealed cyclic changes in functional layer. It was extremely high at late proliferative and early secretary phase, but in basal layer, it was low and revealed no cyclic changes. In endometriosis binding capacities of 8S receptors from the lesions were detected as almost same values, but in some lesions 8S receptors were not detected. In endometrial carcinoma it depended on the degree of differentiation of the tumor. It was high in well differentiated form, and low in undifferentiated form. On well differentiated carcinoma, it was decreased markedly with administration of much gestagen. The other 5S macromolecule was consisted of plasma corticosteroid-binding globulin (plasma CBG) and/or some CBG-like component.
通过葡聚糖包被活性炭分析法，测定了各种子宫内膜胞质溶胶制剂对³H-孕酮的特异性结合能力。蔗糖密度梯度超速离心后，发现了沉降系数为8S和5S的孕酮结合成分。特别是，8S结合成分趋于饱和，且对孕酮具有高度特异性。其解离常数以及与各种未标记类固醇的竞争研究表明，正常和异常子宫内膜之间无显著差异。正常月经周期的子宫内膜中的这种8S受体在功能层呈现周期性变化。在增殖晚期和分泌早期极高，但在基底层则很低且无周期性变化。在子宫内膜异位症中，病变部位8S受体的结合能力检测值几乎相同，但在一些病变中未检测到8S受体。在子宫内膜癌中，这取决于肿瘤的分化程度。高分化型中含量高，未分化型中含量低。在高分化癌中，大量给予孕激素后其含量明显降低。另一种5S大分子由血浆皮质类固醇结合球蛋白（血浆CBG）和/或一些CBG样成分组成。
