Aldskogius H
Neuropathol Appl Neurobiol. 1978 Sep-Oct;4(5):323-41. doi: 10.1111/j.1365-2990.1978.tb01346.x.
Counts of neuronal nucleoli were made in the dorsal motor vagal nucleus (DMV) of the adult rabbit 10, 18, 70 and 90 days following unilateral cervical vagotomy. The structural characteristics of nerve cell bodies in the DMV were studied electron microscopically 2--90 days after cervical vagotomy. The nucleolar counts indicated that 20% of the large DMV neurones had disappeared ipsilateral to the operation 10 days postoperatively (p.o.), 65% 18 days p.o. and 70% 70 and 90 days p.o. No loss of small neurones was found. Large neurones ipsilateral to the operation showed nuclear displacement, infoldings of the nuclear membrane and disappearance of granular endoplasmic reticulum beginning 4 days p.o. and being prominent 6--18 days p.o. At the peak of the response, 10--18 days p.o., reacting neurones showed nucleolar condensation and vacuolation, the appearance of intranuclear electron-dense particles, extensive accumulation of intracytoplasmic lipid droplets, increased numbers of microtubules and neurofilaments, focal mitochondrial aggregates, and widespread mitochondrial degeneration. Ten to 21 days p.o. degenerating neurones were observed. After 30 days p.o. survival a partial recovery of surviving large DMV neurones seemed to have taken place. The findings are interpreted as indications of distubed protein metabolism, oxidative metabolism and intraneuronal transport in the axotomized DMV neurones. The unique response of these neurones compared to previously studied peripherally projecting neurones is emphasized.
在成年兔单侧颈迷走神经切断术后10、18、70和90天，对其迷走神经背核（DMV）中的神经元核仁进行计数。在颈迷走神经切断术后2至90天，通过电子显微镜研究DMV中神经细胞体的结构特征。核仁计数表明，术后10天，手术同侧20%的大型DMV神经元消失；术后18天，消失比例为65%；术后70天和90天，消失比例为70%。未发现小型神经元有损失。手术同侧的大型神经元从术后4天开始出现核移位、核膜折叠以及粗面内质网消失，在术后6至18天最为明显。在反应高峰期，即术后10至18天，反应性神经元表现出核仁凝聚和空泡化、核内电子致密颗粒的出现、胞质内脂质小滴的大量积累、微管和神经丝数量增加、局灶性线粒体聚集以及广泛的线粒体变性。术后10至21天观察到神经元发生退变。术后30天存活时，存活的大型DMV神经元似乎已部分恢复。这些发现被解释为轴突切断的DMV神经元中蛋白质代谢、氧化代谢和神经元内运输受到干扰的迹象。强调了这些神经元与先前研究的外周投射神经元相比的独特反应。
