通过SDS-凝胶过滤柱色谱法分析脂蛋白载脂蛋白

Analysis of lipoprotein apoproteins by SDS-gel filtration column chromatography.

作者信息

Sparks C E, Marsh J B

出版信息

J Lipid Res. 1981 Mar;22(3):514-8.

Abstract

Rat plasma, containing 125I-labeled triglyceride-rich lipoprotein, was mixed following lipid extraction with 10% SDS buffer and analyzed by gel filtration chromatography on columns using an elution buffer containing 1% SDS. Labeled apoproteins were separated into apo B, apo E, and apo C radioactivity peaks. Labeled peptides, tyrosine, and iodide were also resolved by this method. Isolated lipoprotein fractions were separated into the same components. The method offers the advantages of quantitative radioactivity recovery, large sample volume, and resolution of two apo B proteins.

摘要

含有125I标记的富含甘油三酯脂蛋白的大鼠血浆，在脂质提取后与10%十二烷基硫酸钠（SDS）缓冲液混合，并使用含有1% SDS的洗脱缓冲液通过凝胶过滤色谱法在柱上进行分析。标记的载脂蛋白被分离为载脂蛋白B、载脂蛋白E和载脂蛋白C放射性峰。标记的肽、酪氨酸和碘化物也通过该方法得以分离。分离出的脂蛋白组分被分离为相同的成分。该方法具有放射性回收率定量、样品体积大以及能分离两种载脂蛋白B蛋白的优点。