Tai Y H, Tai C Y
J Membr Biol. 1981 Apr 30;59(3):173-7. doi: 10.1007/BF01875423.
The relationships among ion current, membrane potential difference, and resistance of an epithelium are studied. The short-circuit technique introduced by Ussing and Zerahn does not completely short circuit the epithelium if the series resistance parallel to the cell layer between the voltage electrodes is not properly compensated. The residual potential difference across the epithelial cell layer in the "short-circuit state" is proportional to both the measured short-circuited small intestinal mucosa the villus and crypt areas are hypo-polarized to different degrees rather than simultaneously hyper- and hypo-polarized. Short-circuiting the whole tissue reduces but does not abolish the passive net ion movement across the tissue. Measurements of the electrical properties of the whole and denuded rat distal small intestine in HCO3-Ringer solution containing 10 mM glucose reveal that the measured short-circuit current has under-estimated approximately 33% of the true short-circuit current and that the passive net Na flux from serosa to mucosa and Cl flux from mucosa to serosa are not negligible in the "short circuit state."
研究了上皮组织的离子电流、膜电位差和电阻之间的关系。如果未对与电压电极之间的细胞层并联的串联电阻进行适当补偿，那么由乌斯廷和泽拉恩引入的短路技术并不能完全使上皮组织短路。在“短路状态”下，跨上皮细胞层的残余电位差与所测量的短路小肠黏膜成正比，绒毛和隐窝区域以不同程度出现低极化，而非同时出现超极化和低极化。使整个组织短路会减少但不会消除跨组织的被动净离子移动。在含有10 mM葡萄糖的HCO₃ - 林格溶液中对完整的和剥脱的大鼠远端小肠的电特性进行测量，结果表明所测量的短路电流大约低估了真实短路电流的33%，并且在“短路状态”下，从浆膜到黏膜的被动净钠通量以及从黏膜到浆膜的氯通量不可忽略。
