Neiland K A, Miller L G
J Wildl Dis. 1981 Apr;17(2):183-9. doi: 10.7589/0090-3558-17.2.183.
Beagle dogs were readily infected by 1.3 x 10(8) colony forming units (cfu) of Brucella suis type 4 administered either on canned dog food, or intraperitoneally. Such infections were afebrile and otherwise asymptomatic and without any obvious gross lesions. Inoculation of 10(8) cfu B. suis type 4 intraperitoneally into two gravid wolves (Canis lupus) resulted in infections in both animals. About 24 days later they gave birth, apparently at full-term, to two (both alive) and six (two alive and four dead) pups, respectively. Pups born alive died within 24 hours. A black bear (Ursus americanus) infected with between 10(8) and 10(9) cfu yielded serologic and bacteriologic data similar to that derived from the observations on beagles and wolves. Two grizzly bears (Ursus arctos horribilis) were both infected by exposure to 1.3 x 10(9) csf B. suis type 4 placed on canned dog food. Antibody titres reached very high levels within the first two months of infection.
比格犬很容易被4型猪布鲁氏菌的1.3×10⁸菌落形成单位（cfu）感染，这些菌通过罐装狗粮喂食或腹腔注射给药。此类感染不发热，也无其他症状，且无任何明显的肉眼可见病变。给两只怀孕的狼（犬属）腹腔注射10⁸cfu的4型猪布鲁氏菌，两只动物均被感染。大约24天后，它们分别足月产下两只幼崽（均存活）和六只幼崽（两只存活，四只死亡）。存活出生的幼崽在24小时内死亡。一只感染了10⁸至10⁹cfu的黑熊（美洲黑熊）产生的血清学和细菌学数据与对比格犬和狼的观察结果相似。两只灰熊（棕熊）均通过接触放置在罐装狗粮上的1.3×10⁹cfu的4型猪布鲁氏菌而被感染。在感染的前两个月内，抗体滴度达到非常高的水平。
