Hymer W C, Wilbur D L, Page R, Hibbard E, Kelsey R C, Hatfield J M
Neuroendocrinology. 1981 Jun;32(6):339-49. doi: 10.1159/000123184.
A new technique is described for the study of mammalian pituitary cells in vivo and in vitro. The method involves encapsulation of freshly trypsinized rat, sheep or human pituitary cells in XM-50 Amicon hollow fibers followed by their intracranial implantation into hypophysectomized rats. These pituitary fiber units promoted recipient growth for 3 weeks before weight gains plateaued. Body composition analyses showed that significant quantities of protein, fat and ash accounted for the weight gain. Morphological study of the capsule contents 7-39 days postimplantation revealed the presence of intact somatotrophs and corticotrophs. The hollow fibers may provide an immunologically privileged site by virtue of the fact that the 50,000 dalton pores making up the lumen surface permit pituitary hormones to diffuse from the capsule, but theoretically do not permit immunoglobulin molecules to penetrate the capsule. Growth of hypox rats receiving capsules containing allogeneic rat pituitary cells, sheep cells or pieces of human postmortem pituitary support this concept. Furthermore, rats implanted with human PRL adenoma cells had detectable quantities of circulating hPRL 100 days postimplantation. It is suggested that the pituitary hollow fiber units function when they come in contact with a ventricular surface of a hypox animal. With these units, it will be possible to study function of the same group of pituitary cells in vitro and in vivo.
本文描述了一种用于研究哺乳动物垂体细胞体内和体外情况的新技术。该方法包括将新鲜胰蛋白酶消化后的大鼠、绵羊或人类垂体细胞包裹在XM - 50 Amicon中空纤维中，然后将其颅内植入垂体切除的大鼠体内。这些垂体纤维单元在受体体重增加趋于平稳之前促进其生长达3周。身体成分分析表明，体重增加主要是由于大量的蛋白质、脂肪和灰分。植入后7 - 39天对胶囊内容物的形态学研究显示存在完整的生长激素细胞和促肾上腺皮质激素细胞。中空纤维可能提供一个免疫特惠部位，因为构成管腔表面的50,000道尔顿的孔隙允许垂体激素从胶囊中扩散出来，但理论上不允许免疫球蛋白分子穿透胶囊。接受含有同种异体大鼠垂体细胞、绵羊细胞或人类垂体死后切片胶囊的垂体切除大鼠的生长情况支持了这一概念。此外，植入人类催乳素腺瘤细胞的大鼠在植入后100天可检测到循环中的人催乳素。有人提出，垂体中空纤维单元在与垂体切除动物的脑室表面接触时发挥作用。利用这些单元，将有可能在体外和体内研究同一组垂体细胞的功能。
