Holst E, Helin I, Mårdh P A
Scand J Infect Dis. 1981;13(1):41-5. doi: 10.1080/00365548.1981.11690365.
The occurrence of Clostridium difficile in faecal specimens of 218 children, aged 2 weeks to 15 years, was studied. The organism was recovered from 43 (20%) of the children (range 2 weeks to 10 years). The isolation frequency was significantly correlated to age. Thus, in children 1 to 8 months of age the organism occurred in 64%, while in children below and above that age C. difficile could only be recovered in 4%. No significant difference in the recovery frequency could be demonstrated between children with (23%) and without (17%) gastroenteritis. C. difficile occurred numerically more often in non-antibiotic treated children (22%) than in those given such drugs (13%). None of the children in the present study had evidence of pseudomembranous colitis. A comparative study of different selective media did not demonstrate any difference in the recovery frequency of C. difficile. The media used were Chopped Meat Glucose broth with cycloserine and either kanamycin or cefoxitin, and Cycloserine-Cefoxitin-Fructose agar.
对218名年龄在2周至15岁儿童的粪便样本中艰难梭菌的出现情况进行了研究。在43名（20%）儿童（年龄范围为2周至10岁）的样本中分离出了该菌。分离频率与年龄显著相关。因此，在1至8个月大的儿童中，该菌的出现率为64%，而在该年龄以下和以上的儿童中，艰难梭菌的分离率仅为4%。患肠胃炎的儿童（23%）和未患肠胃炎的儿童（17%）之间的分离频率无显著差异。未接受抗生素治疗的儿童（22%）中艰难梭菌的出现率在数量上高于接受此类药物治疗的儿童（13%）。本研究中的儿童均无伪膜性结肠炎的证据。对不同选择性培养基的比较研究未显示艰难梭菌分离频率有任何差异。所使用的培养基为含环丝氨酸和卡那霉素或头孢西丁的碎肉葡萄糖肉汤，以及环丝氨酸-头孢西丁-果糖琼脂。
