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评估环丝氨酸头孢西丁果糖琼脂（CCFA）、含马血和牛胆酸钠的 CCFA 以及含牛胆酸钠和溶菌酶的环丝氨酸头孢西丁甘露醇肉汤，用于从粪便样本中回收艰难梭菌分离株。

Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.

机构信息

RM Alden Research Laboratory, Culver City, California, USA.

出版信息

J Clin Microbiol. 2013 Sep;51(9):3094-6. doi: 10.1128/JCM.00879-13. Epub 2013 Jun 26.

DOI:10.1128/JCM.00879-13
PMID:23804392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3754669/
Abstract

Cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate (CCFA-HT), and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared for recovery of Clostridium difficile from 120 stool specimens. Compared to CCFA, CCFA-HT enhanced C. difficile growth and improved recovery by 4%. In a separate study, 9% (8/91) of stool samples previously C. difficile negative on plate medium were C. difficile positive when cultured in CCMB-TAL.

摘要

环丝氨酸头孢西丁果糖琼脂（CCFA）、添加马血和牛胆盐的 CCFA（CCFA-HT），以及添加牛胆盐和溶菌酶的环丝氨酸头孢西丁甘露醇肉汤（CCMB-TAL）用于从 120 份粪便标本中回收艰难梭菌。与 CCFA 相比，CCFA-HT 增强了艰难梭菌的生长，回收率提高了 4%。在另一项研究中，9%（8/91）先前在平板培养基上艰难梭菌阴性的粪便样本在 CCMB-TAL 中培养时呈艰难梭菌阳性。

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Evaluation of cycloserine-cefoxitin fructose agar (CCFA), CCFA with horse blood and taurocholate, and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme for recovery of Clostridium difficile isolates from fecal samples.评估环丝氨酸头孢西丁果糖琼脂（CCFA）、含马血和牛胆酸钠的 CCFA 以及含牛胆酸钠和溶菌酶的环丝氨酸头孢西丁甘露醇肉汤，用于从粪便样本中回收艰难梭菌分离株。
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