利用小角和广角X射线散射以及圆二色性研究DNA与精胺的相互作用。
Study of DNA-spermine interactions by use of small-angle and wide-angle X-ray scattering and circular dichroism.
作者信息
Damaschun H, Damaschun G, Becker M, Buder E, Misselwitz R, Zirwer D
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3801-9. doi: 10.1093/nar/5.10.3801.
Abstract
Circular dichroism measurements with DNA-spermine complexes at 0.075 M NaCl and at 0.15 M NaCl reveal +psi (type I) and -psi (type II) CD spectra respectively. From small-angle X-ray scattering studies it could be shown that type I has a long-range order, short-range order supramolecular structure, while type II is of long-range disorder, short-range disorder structure. The secondary structure of the DNA in both types of condensates is B-like as concluded from wide-angle X-ray scattering diagrams of the condensates and from a comparison with the wide-angle X-ray curves of DNA and RNA in solution.
摘要
在0.075M NaCl和0.15M NaCl条件下对DNA-精胺复合物进行圆二色性测量，分别得到了+ψ（I型）和-ψ（II型）的圆二色光谱。从小角X射线散射研究可以看出，I型具有长程有序、短程有序的超分子结构，而II型则是长程无序、短程无序的结构。从凝聚物的广角X射线散射图以及与溶液中DNA和RNA的广角X射线曲线比较得出，两种凝聚物中DNA的二级结构均为B型。
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