精胺 - DNA 复合物形成亚稳结构。小角 X 射线散射和圆二色性研究。
Spermine-DNA complexes build up metastable structures. Small-angle X-ray scattering and circular dichroism studies.
作者信息
Becker M, Misselwitz R, Damaschun H, Damaschun G, Zirwer D
出版信息
Nucleic Acids Res. 1979 Nov 10;7(5):1297-309. doi: 10.1093/nar/7.5.1297.
Abstract
Spermine-DNA complexes have been examined by small-angle and wide-angle X-ray scattering as well as by circular dichroism studies. Condensed complexes are building up below a critical ionic strength. We have found that at one and the same ionic strength condensed complexes having two different supramolecular structures (Type I and Type II) can coexist. The structure of the condensates depends on the method of condensate formation. Phase transitions between these structures can be induced by thermal treatment. We conclude from these facts that with polyamine-DNA condensates metastable structures are of importance.
摘要
已通过小角和广角X射线散射以及圆二色性研究对精胺 - DNA复合物进行了检测。在临界离子强度以下会形成凝聚复合物。我们发现，在相同的离子强度下，具有两种不同超分子结构（I型和II型）的凝聚复合物可以共存。凝聚物的结构取决于凝聚物的形成方法。这些结构之间的相变可通过热处理诱导。从这些事实我们得出结论，对于多胺 - DNA凝聚物，亚稳结构很重要。
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