双链RNA真菌病毒颗粒中的半保留转录
Semi-conservative transcription in particles of a double-stranded RNA mycovirus.
作者信息
Ratti G, Buck K W
出版信息
Nucleic Acids Res. 1978 Oct;5(10):3843-54. doi: 10.1093/nar/5.10.3843.
Abstract
During transcription in vitro catalysed by the virion RNA polymerase of Aspergillus foetidus virus AfV-S in the presence of tritiated UTP, the virus double-stranded RNA becomes labelled in one strand, which has the same sequence as the single-stranded RNA transcripts produced. Most of the label incorporated into double-stranded RNA could be chased into single stranded RNA by further reaction with excess unlabelled nucleoside triphosphates. In reactions containing tritiated UTP the single-stranded RNA transcripts released after the first round of transcription were unlabelled. It is concluded that transcription in virions of AfV-S occurs by displacement of one of the strands of double-stranded RNA by the RNA strand being newly synthesised i.e. the reaction is semi-conservative with respect to double-stranded RNA.
摘要
在存在氚标记的三磷酸尿苷的情况下，由臭曲霉病毒AfV-S的病毒粒子RNA聚合酶催化进行体外转录时，病毒双链RNA的一条链被标记，该链与产生的单链RNA转录本具有相同序列。通过与过量未标记的核苷三磷酸进一步反应，掺入双链RNA中的大部分标记物可被追踪到单链RNA中。在含有氚标记的三磷酸尿苷的反应中，第一轮转录后释放的单链RNA转录本未被标记。得出的结论是，AfV-S病毒粒子中的转录是通过新合成的RNA链取代双链RNA的一条链来进行的，即就双链RNA而言，该反应是半保留的。
相似文献
1
Semi-conservative transcription in particles of a double-stranded RNA mycovirus.双链RNA真菌病毒颗粒中的半保留转录
Nucleic Acids Res. 1978 Oct;5(10):3843-54. doi: 10.1093/nar/5.10.3843.
2
Semi-conservative transcription of double-stranded RNA catalyzed by bacteriophage phi 6 RNA polymerase.噬菌体φ6 RNA聚合酶催化的双链RNA半保留转录
J Biochem. 1980 Dec;88(6):1569-75. doi: 10.1093/oxfordjournals.jbchem.a133131.
3
A novel dsRNA element isolated from the Aspergillus foetidus mycovirus complex.从产黄青霉真菌病毒复合体中分离出的一种新型 dsRNA 元件。
Arch Virol. 2013 Dec;158(12):2625-8. doi: 10.1007/s00705-013-1779-3. Epub 2013 Jul 5.
4
Transcribing and replicating particles in a double-stranded RNA virus from Leishmania.来自利什曼原虫的双链RNA病毒中的转录和复制颗粒
Mol Biochem Parasitol. 1992 Jun;52(2):207-13. doi: 10.1016/0166-6851(92)90053-m.
5
Genomic structure and RNA polymerase activity in Leishmania virus.利什曼病毒的基因组结构与RNA聚合酶活性
J Virol. 1991 Aug;65(8):4211-5. doi: 10.1128/JVI.65.8.4211-4215.1991.
6
Conservative replication and transcription of Saccharomyces cerevisiae viral double-stranded RNA in vitro.酿酒酵母病毒双链RNA在体外的保守复制与转录
J Virol. 1986 Mar;57(3):754-8. doi: 10.1128/JVI.57.3.754-758.1986.
7
RNA polymerase activity in virions from Ustilago maydis.来自玉米黑粉菌的病毒粒子中的RNA聚合酶活性。
Mol Cell Biol. 1984 Jan;4(1):188-94. doi: 10.1128/mcb.4.1.188-194.1984.
8
Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus.从aspergillus foetidus 中分离出的一种四分体 dsRNA 病毒的序列测定。
Arch Virol. 2013 Jan;158(1):267-72. doi: 10.1007/s00705-012-1362-3. Epub 2012 Jul 4.
9
Native replication intermediates of the yeast 20 S RNA virus have a single-stranded RNA backbone.酵母20S RNA病毒的天然复制中间体具有单链RNA骨架。
J Biol Chem. 2005 Feb 25;280(8):7398-406. doi: 10.1074/jbc.M412048200. Epub 2004 Dec 14.
10
Transcription of killer virion double-stranded RNA in vitro.杀伤性病毒粒子双链RNA的体外转录
Nucleic Acids Res. 1980 Jun 11;8(11):2365-75. doi: 10.1093/nar/8.11.2365.
引用本文的文献
1
RNA-Dependent RNA Polymerase from Heterobasidion RNA Virus 6 Is an Active Replicase In Vitro.异担子 RNA 病毒 6 的 RNA 依赖性 RNA 聚合酶在体外是一种有活性的复制酶。
Viruses. 2021 Aug 31;13(9):1738. doi: 10.3390/v13091738.
2
RdRp or RT, That is the Question.RdRp 还是 RT，这是个问题。
Mol Biol Evol. 2021 Oct 27;38(11):5082-5091. doi: 10.1093/molbev/msab235.
3
Mycoviruses in : A Comprehensive Review.《中的真菌病毒：全面综述》 （你提供的原文不完整，推测标题可能是这样，具体需结合完整内容确定准确翻译）
Front Microbiol. 2017 Sep 6;8:1699. doi: 10.3389/fmicb.2017.01699. eCollection 2017.
4
3D structures of fungal partitiviruses.真菌二分体病毒的 3D 结构。
Adv Virus Res. 2013;86:59-85. doi: 10.1016/B978-0-12-394315-6.00003-9.
5
Effects of viscogens on RNA transcription inside reovirus particles.粘弹性物质对呼肠孤病毒粒子内 RNA 转录的影响。
J Biol Chem. 2011 Aug 26;286(34):29521-30. doi: 10.1074/jbc.M111.241703. Epub 2011 Jun 30.
6
Probing the transcription mechanisms of reovirus cores with molecules that alter RNA duplex stability.用改变RNA双链稳定性的分子探究呼肠孤病毒核心的转录机制。
J Virol. 2009 Jun;83(11):5659-70. doi: 10.1128/JVI.02192-08. Epub 2009 Mar 18.
7
Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.噬菌体phi 6 RNA聚合酶对单链RNA的半保留合成
J Virol. 1980 Feb;33(2):769-73. doi: 10.1128/JVI.33.2.769-773.1980.
8
Replication of double-stranded RNA of the virus-like particles in Saccharomyces cerevisiae.病毒样颗粒的双链RNA在酿酒酵母中的复制。
J Virol. 1981 Apr;38(1):263-71. doi: 10.1128/JVI.38.1.263-271.1981.
9
Yeast viral RNA polymerase is a transcriptase.酵母病毒RNA聚合酶是一种转录酶。
Nucleic Acids Res. 1980 Jul 11;8(13):2985-97. doi: 10.1093/nar/8.13.2985.
10
Conservative replication and transcription of Saccharomyces cerevisiae viral double-stranded RNA in vitro.酿酒酵母病毒双链RNA在体外的保守复制与转录
J Virol. 1986 Mar;57(3):754-8. doi: 10.1128/JVI.57.3.754-758.1986.
本文引用的文献
1
INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI.大肠杆菌中染色体起源处胸腺嘧啶饥饿诱导复制
J Mol Biol. 1964 Aug;9:288-307. doi: 10.1016/s0022-2836(64)80208-4.
2
Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.呼肠孤病毒相关的依赖核糖核酸的聚合酶的体外转录
J Virol. 1970 Jul;6(1):1-11. doi: 10.1128/JVI.6.1.1-11.1970.
3
Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.呼肠孤病毒核心催化的呼肠孤病毒RNA体外转录研究
Virology. 1969 Dec;39(4):822-31. doi: 10.1016/0042-6822(69)90019-1.
4
RNA polymerase activity associated with bacteriophage phi 6.与噬菌体φ6相关的RNA聚合酶活性
J Virol. 1973 Sep;12(3):464-71. doi: 10.1128/JVI.12.3.464-471.1973.
5
Synthesis of reovirus double-stranded RNA within virionlike particles.呼肠孤病毒双链RNA在病毒样颗粒内的合成。
Virology. 1972 Nov;50(2):349-58. doi: 10.1016/0042-6822(72)90386-8.
6
Biophysical studies of reovirus type 3. III. A laser light-scattering study of the RNA transcriptase reaction.
Virology. 1976 Mar;70(1):28-36. doi: 10.1016/0042-6822(76)90233-6.
7
Replication of double-stranded RNA in particles of Penicillium stoloniferum virus S.匐枝青霉病毒S颗粒中双链RNA的复制
Nucleic Acids Res. 1975 Oct;2(10):1889-902. doi: 10.1093/nar/2.10.1889.
8
RNA polymerase activity in double-stranded ribonucleic acid virus particles from Aspergillus foetidus.来自臭曲霉的双链核糖核酸病毒颗粒中的RNA聚合酶活性。
Biochem Biophys Res Commun. 1975 Sep 16;66(2):706-11. doi: 10.1016/0006-291x(75)90567-7.
9
Intermediates in the biosynthesis of double-stranded ribonucleic acids of bacteriophage phi 6.噬菌体φ6双链核糖核酸生物合成的中间体
Proc Natl Acad Sci U S A. 1975 Mar;72(3):849-53. doi: 10.1073/pnas.72.3.849.
10
Molecular weight of double-stranded RNA: a re-examination of Aspergillus foetidus virus S RNA components.双链RNA的分子量：对臭曲霉病毒S RNA组分的重新检测
J Gen Virol. 1977 Oct;37(1):215-9. doi: 10.1099/0022-1317-37-1-215.
Zotero 插件