Marzluff W F, Pan C J, Cooper D L
Nucleic Acids Res. 1978 Nov;5(11):4177-93. doi: 10.1093/nar/5.11.4177.
Myeloma cells have been synchronized by isoleucine starvation. Changes in RNA synthetic rates as a result of starvation have been studied. The ability of isolated nuclei to synthesize RNA declines on starvation and increases subsequently on refeeding isoleucine. There is a coordinate drop in synthetic rate for all three polymerases both in vivo and in vitro. The chain elongation rate in vitro is the same in starved and normal cells, so the difference is in the number of active polymerases in vitro. However, the nuclei do not exactly parallel the state of the cell from which they were isolated, but the in vitro RNA synthesis increases more slowly than the in vivo RNA synthesis. There is no change in relative amounts of synthesis by the different RNA polymerases. The in vitro RNA product is similar in starved and growing cells.
骨髓瘤细胞已通过异亮氨酸饥饿实现同步化。研究了饥饿导致的RNA合成速率变化。分离的细胞核合成RNA的能力在饥饿时下降，随后在重新添加异亮氨酸时增加。体内和体外所有三种聚合酶的合成速率均协同下降。饥饿细胞和正常细胞体外的链延伸速率相同，因此差异在于体外活性聚合酶的数量。然而，细胞核与分离它们的细胞状态并不完全平行，体外RNA合成的增加比体内RNA合成更缓慢。不同RNA聚合酶的相对合成量没有变化。饥饿细胞和生长细胞的体外RNA产物相似。
