Mauck J C, Green H
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2819-22. doi: 10.1073/pnas.70.10.2819.
Addition of serum, containing fibroblast growth factors, to a culture of resting 3T6 cells stimulates a transition to the growing state. Studies of ghost monolayers prepared with the aid of detergent at intervals after stimulation showed an increase in the rate of ribosomal RNA synthesis within 10 min. The rate continued to increase for many hours and reached a level 2.5- to 3.5-fold higher by the time DNA synthesis began. The increasing rate of ribosomal RNA synthesis appeared independent of an increase in the number of ribosomal genes, since it was not affected by prevention of DNA synthesis with cytosine arabinoside. In contrast to ribosomal RNA, the overall rate of transscription of heterogeneous nuclear RNA was not directly affected by serum growth factors and does not appear to be regulated during the transition from resting to growing state. It seems, instead, to be fixed in relation to the amount of template, for it increases proportionally to DNA content.
向静止的3T6细胞培养物中添加含有成纤维细胞生长因子的血清会刺激细胞转变为生长状态。在刺激后的不同时间间隔，借助去污剂制备的空壳单层细胞研究表明，核糖体RNA合成速率在10分钟内增加。该速率持续增加数小时，到DNA合成开始时达到高出2.5至3.5倍的水平。核糖体RNA合成速率的增加似乎与核糖体基因数量的增加无关，因为它不受阿糖胞苷抑制DNA合成的影响。与核糖体RNA相反，不均一核RNA的总体转录速率不受血清生长因子的直接影响，并且在从静止状态转变为生长状态的过程中似乎不受调控。相反，它似乎与模板量相关固定，因为它与DNA含量成比例增加。
