Cohen E J, Allansmith M R
Am J Ophthalmol. 1981 Jun;91(6):789-93. doi: 10.1016/0002-9394(81)90014-3.
We compared biopsy specimens of human lacrimal gland and conjunctiva preserved by alcohol-acetic acid with the same tissues preserved by freezing to find secretory component. Secretory component was found in lacrimal gland preserved both by alcohol-acetic acid and by freezing. The structure of the tissue was better preserved with the alcohol-acetic acid fixation. Three of eight conjunctival specimens preserved by alcohol-acetic acid showed weak staining for secretory component in a few superficial epithelial cells. We found nonspecific staining of the conjunctiva with the control antiserum, rhodamine-labeled goat antibovine serum. Two specimens preserved by freezing were unreadable; four of the remaining six stained nonspecifically with the remaining two specimens stained with neither the control nor the experimental antiserum; the second may have had more staining with the experimental antiserum than with the control. We found no evidence for including the conjunctiva in the secretory IgA immune system.
我们比较了用酒精 - 乙酸保存的人泪腺和结膜活检标本与用冷冻保存的相同组织，以寻找分泌成分。在经酒精 - 乙酸和冷冻保存的泪腺中均发现了分泌成分。酒精 - 乙酸固定能更好地保存组织结构。八份经酒精 - 乙酸保存的结膜标本中有三份在少数表层上皮细胞中显示出分泌成分的弱阳性染色。我们发现对照抗血清（罗丹明标记的山羊抗牛血清）对结膜有非特异性染色。两份经冷冻保存的标本无法读取；其余六份标本中有四份与对照抗血清有非特异性染色，另外两份标本既不与对照抗血清也不与实验抗血清染色；第二份标本与实验抗血清的染色可能比与对照抗血清的染色更多。我们没有发现结膜参与分泌型IgA免疫系统的证据。
