利用万古霉素分离耻垢分枝杆菌的特定营养缺陷型突变体。
Isolating specific auxotrophic mutants of Mycobacterium smegmatis by using vancomycin.
作者信息
Woodley C L, Baldwin J N, Greenberg J
出版信息
Antimicrob Agents Chemother. 1981 Apr;19(4):571-4. doi: 10.1128/AAC.19.4.571.
Abstract
After inducing mutagenesis with nitrosoguanidine, we used vancomycin enrichment in isolating auxotrophic mutants of Mycobacterium smegmatis. Compared with cycloserine and penicillin G, which are also cell wall inhibitors, vancomycin, with 24 h of exposure, produced less cell lysis and a greater reduction in viable cell numbers. With vancomycin enrichment, 13 specific auxotrophs were isolated after nitrosoguanidine-induced mutation, whereas only two mutants were isolated without enrichment.
摘要
在用亚硝基胍诱变后，我们使用万古霉素富集法来分离耻垢分枝杆菌的营养缺陷型突变体。与同样是细胞壁抑制剂的环丝氨酸和青霉素G相比，暴露24小时的万古霉素导致的细胞裂解较少，活细胞数量减少得更多。通过万古霉素富集法，在亚硝基胍诱导突变后分离出了13种特定的营养缺陷型，而未进行富集时仅分离出了两种突变体。
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A procedure for selecting and isolating specific auxotrophic mutants of Mycobacterium smegmatis.一种用于选择和分离耻垢分枝杆菌特定营养缺陷型突变体的方法。
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